Plant Total RNA Isolation Plus Kit
Bayanin Kit:
Cire DNA yadda yakamata ta amfani da Rukunin Tsabtace DNA
Cire DNA ba tare da ƙara DNA ba
Sauƙaƙan-dukkan ayyukan ana kammala su a cikin zafin jiki
Ana iya kammala aiki mai sauri a cikin mintuna 30
Amintacciya — ba a yi amfani da reagent na halitta ba
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 200
Kit ɗin yana amfani da ginshiƙin juzu'i da dabara wanda Foregene ya haɓaka, wanda zai iya fitar da inganci mai inganci da ingantaccen jimlar RNA daga kyallen shuka iri-iri tare da babban polysaccharides ko abun ciki na polyphenols.Yana ba da ginshiƙin Tsabtace DNA wanda zai iya cire DNA na genomic cikin sauƙi daga lysate mai girma da nama.Rukunin RNA-kawai zai iya ɗaure RNA daidai yadda ya kamata.Kit ɗin na iya aiwatar da babban adadin samfurori a lokaci guda.
Duk tsarin bai ƙunshi RNase ba, don haka tsarkakewar RNA ba zai ƙasƙanta ba.Buffer PRW1 da Buffer PRW2 na iya tabbatar da cewa RNA da aka samu ba ta gurɓata su ta hanyar furotin, DNA, ions, da mahadi na halitta ba.
Abubuwan Kit
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Free ddH2O, Rukunin Tsabtace DNA |
| Rukunin RNA-Kawai |
Fasaloli & fa'idodi
■ Aiki a dakin da zafin jiki (15-25 ℃) a ko'ina cikin tsari, ba tare da wanka na kankara da ƙananan zafin jiki ba.
n Cikakken kayan aikin RNase-Free, babu buƙatar damuwa game da lalata RNA.
Musamman dacewa don tsarkakewar RNA daga samfuran shuka na polysaccharides da polyphenols.
■ Rukunin Tsabtace DNA yana ɗaure musamman ga DNA, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara DNA ba.
∎ Babban yawan amfanin RNA: Rukunin RNA-kawai da tsari na musamman na iya tsarkake RNA da kyau.
n Gudun sauri: mai sauƙin aiki kuma ana iya kammala shi cikin mintuna 30.
∎ Tsaro: ba a bužatar sinadarin reagents.
∎ Babban inganci: gutsuttssun RNA da aka tsarkake suna da tsafta, ba su da furotin da sauran ƙazanta, kuma suna iya saduwa da aikace-aikacen gwaji daban-daban.
Siffofin samfur
∎ Aikace-aikace na ƙasa: haɗin cDNA na farko, RT-PCR, cloning na kwayoyin halitta, Northern Blot, da sauransu.
Misali: Sabbin kyallen tsire-tsire ko daskararre na polysaccharides da polyphenols
■ Adadin: 50mg nama na shuka
n Matsakaicin ƙarfin ɗaurin RNA na ginshiƙin tsarkakewa: 80 μg
■ Ƙarfin haske: 50-200 μl
Kit aikace-aikace
Ya dace da hakar da tsarkakewa na jimillar RNA daga samfuran nama mai sabo ko daskararre (musamman sabobin ganyayyakin ganyayyaki) tare da babban polysaccharide da abun ciki na polyphenol.
Gudun aiki
zane
Plant Total RNA Isolation Kit Plus ya sarrafa 50mg na sabobin ganye na polysaccharides da polyphenols, kuma 5% tsarkakewar RNA an gwada ta hanyar electrophoresis.
1: banana
2: ginkgo
3: auduga
4: Ruman
Adana da Rayuwar Shelf
Ana iya adana wannan kit ɗin na tsawon watanni 24 a ƙarƙashin yanayin bushewa a cikin zafin jiki (15-25 ℃);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a cikin 2-8 ℃.
Buffer PSL1 za a iya sanya shi a 4 ℃ na wata 1 bayan ƙara β-mercaptoethanol (an bada shawarar ƙara shi a lokaci guda na gwaji).
