Kayan Naman Dabbobi Kai tsaye PCR kit-UNG (ba tare da Kayan Samfura ba) yarjejeniya
Bayanin Kit:
Yi amfani da lysate nama na dabba kai tsaye azaman samfuri don haɓaka PCR, ba tare da warewar DNA daban ba, sauri da hankali mai girma.An ƙara tsarin hana gurɓacewar yanayi na UNG don kawar da abubuwan da ba su dace ba.
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.
◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.
Bayani
Wannan kit ɗin yana amfani da tsarin buffer na lysis na musamman don sakin DNA da sauri daga samfuran nama na dabba don halayen PCR, don haka ya dace musamman don gwajin ƙwayoyin cuta masu girma.
An kammala aiwatar da sakin kwayoyin halittar DNA daga ma'aunin lysis a cikin mintuna 10-30 a 65°C.Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma za a iya amfani da DNA ɗin da aka saki azaman samfuri don amsawar PCR.
2 × PCR Mai SauƙiTMMix (UNG) yana amfani da dUTP maimakon dTTP akan 2 × PCR EasyTMMix, kuma ƙara UNG enzyme (Uracil-N-glycosylase) wanda zai iya lalata samfurin da ke ɗauke da dUTP a lokaci guda.Kafin amsawar PCR, ana amfani da enzyme na UNG don lalata samfurin PCR mai ɗauke da uracil.Enzyme na UNG ba zai yi wani tasiri akan samfurin da ba ya ƙunshi uracil, don haka tabbatar da ƙayyadaddun ƙayyadaddun ƙayyadaddun haɓakawa da kuma hana yuwuwar gurɓatar samfuran PCR yayin gwajin ƙwayoyin cuta masu girma.
D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.
Abubuwan Kit
| Kashi na I | Buffer AL |
| Maganin Protease | |
| 6× DNA Loading Buffer | |
| Kashi na II | 2 × PCR Mai SauƙiTMMix(UNG) |
Fasaloli & fa'idodi
■Babu tsarkakewar DNA mai cin lokaci da tsada
∎ Bukatar samfurin karami ne, kamar yadda 5mg za a iya gwada nama na dabba.
■ Ba a buƙatar magani na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.
2 Tsarin PCR Anti-Pollution×PCR SauƙiTMMix (UNG) yadda ya kamata yana kawar da gurbatar yanayi da samfuran PCR ke haifarwa kuma yana tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Kayan aiki sigogi
Aikace-aikace: nau'ikan kyallen takarda na dabba.
DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.
Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana sashe na I na wannan kit ɗin a 2-8℃.
Ana iya adana buffer AL reagent na watanni 12 a ƙarƙashin bushewa;ana iya adana shi a cikin 2-8℃don dogon ajiya.
Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aikinsa da kwanciyar hankali, da fatan za a adana shi a 4℃na watanni 12.
Reagen 6×Ana iya adana buffer Loading DNA a 4℃ko -20℃na dogon lokaci.
Ya kamata a adana Sashe na II na wannan kit a -20℃.
Reagent 2×PCR SauƙiTMMix (UNG), idan ana amfani dashi akai-akai, ana iya adana shi a 4℃don ajiya na ɗan gajeren lokaci (amfani da sama a cikin kwanaki 10).
