Kit ɗin PCR kai tsaye na Tissue na dabba (ba tare da Samfuran Kayan aikin ba) yarjejeniya
Bayanin Kit:
Yi amfani da lysate nama na dabba kai tsaye azaman samfuri don haɓaka PCR, ba tare da warewar DNA daban ba, sauri da hankali mai girma.
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.
◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.
Bayani
Wannan kit ɗin yana amfani da tsarin buffer na lysis na musamman don sakin DNA da sauri daga samfuran nama na dabba don halayen PCR, don haka ya dace musamman don gwajin ƙwayoyin cuta masu girma.
An kammala aiwatar da sakin kwayoyin halittar DNA daga ma'aunin lysis a cikin mintuna 10-30 a 65°C.Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma za a iya amfani da DNA ɗin da aka fitar azaman samfuri don amsawar PCR.
2 × PCR Mai SauƙiTMMix yana da ƙarfi mai ƙarfi ga masu hanawa na PCR, kuma yana iya amfani da lysate na samfurin don gwadawa azaman samfuri don ingantaccen haɓakawa da takamaiman.Wannan reagen ya ƙunshi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, amsawa buffer, PCR ingantawa da stabilizer.
D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
| Kashi na I | Buffer AL |
| Maganin Protease | |
| 6× DNA Loading Buffer | |
| Kashi na II | 2 × PCR Mai SauƙiTMMix |
Fasaloli & fa'idodi
■Babu tsarkakewar DNA mai cin lokaci da tsada
∎ Bukatar samfurin karami ne, kamar yadda 5mg za a iya gwada nama na dabba.
■ Ba a buƙatar magani na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.
Kayan aiki sigogi
Aikace-aikace: nau'ikan kyallen takarda na dabba.
DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.
Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana sashe na I na wannan kit ɗin a 2-8℃.
Ana iya adana buffer AL reagent na watanni 12 a ƙarƙashin bushewa;ana iya adana shi a cikin 2-8℃don dogon ajiya.
Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aikinsa da kwanciyar hankali, da fatan za a adana shi a 4℃na watanni 12.
Reagen 6×Ana iya adana buffer Loading DNA a 4℃ko -20℃na dogon lokaci.
Ya kamata a adana Sashe na II na wannan kit a -20℃.
Reagent 2×PCR SauƙiTMAna iya adana cakuda don watanni 12 a -20℃;idan ana amfani dashi akai-akai, ana iya adana shi a 4℃don ajiya na ɗan gajeren lokaci (amfani da sama a cikin kwanaki 10).
