Kayan shuka kai tsaye PCR Plus Kit (ba tare da Kayan Samfuri ba) Protocol Direct PCR daga Kayan Shuka
Bayani
Wannan samfurin yana amfani da tsarin buffer na musamman don lyse ganyen shuka.Ana iya amfani da lysate azaman samfuri ba tare da tsarkakewa ba.Bayan ƙara abubuwan farko, PCR Mix na wannan kit ɗin za a iya amfani dashi don haɓakawa.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
Kashi na I | Buffer PS1 |
Buffer P2 | |
6×Mai Buffer Loading DNA | |
Kashi na II | Buffer PS2 |
2× Leaf PCR EasyTMMix |
Fasaloli & fa'idodi
Babu tsarkakewar DNA mai cin lokaci da tsada
■ Ƙananan abu
∎ Sauƙaƙe-samfurin za a iya jujjuya shi zuwa amsawar PCR ko amsawar lysis ba tare da yanke ko niƙa ba
∎ 2 × Mix, rage kuskuren ɗorawa samfurin da lokacin shirye-shiryen tsarin amsawa
∎ Za a iya kammala shirye-shiryen samfuri cikin sauri cikin mintuna 10, ana iya kammala aikin PCR cikin aƙalla mintuna 50.
∎ Za'a iya kammala amsawar ɗimbin kayan aiki mai girma a cikin farantin PCR mai rijiyar 96
Kayan aiki sigogi
Aikace-aikace: Gane na transgenic shuke-shuke, genotyping, da dai sauransu.
Misali: ganyen shuka mai wadata a polysaccharide ko polyphenol
Girman samfurin: Diamita 2-3mm (hanyar kai tsaye), diamita 5-7mm (hanyar lysis)
Kewayon ganowa: PCR samfur ≤1kb ku