Blood SuperDirectᵀᴹ PCR Kit (UNG) -EDTA Jini Kai tsaye PCR Master Mix don Genotyping na Jini
Bayanin Kit:
Kai tsaye yi amfani da EDTA gabaɗayan jinin da ke maganin ƙwanƙwasa a matsayin samfuri don haɓakawa na PCR, tare da babban hankali da ingantaccen haɓakawa.An kara tsarin hana gurbatar yanayi na UNG don hana abubuwan da ba su dace ba.
◮Babu buƙatar tsarkakewar DNA mai cin lokaci da tsada, babu pretreatment, da amfani da jini kai tsaye azaman samfuri na PCR.
◮Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini a hankali.
◮2× SuperEasyTMMix (UNG) - EDTA yana da babban jurewar jini: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
◮Samfurin yana rufe cikakke, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.Tsarin PCR na rigakafi
◮2×SuperEasyTMMix (UNG) -EDTA, yadda ya kamata yana kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa, kuma yana tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Ƙayyadaddun bayanai
200 × 20μl rxns, 2000 × 20μl rxns
2× SuperEasyTMMix (UNG) -EDTA: A kan tushen 2 × SuperEasyTMMix-EDTA, ana amfani da dUTP maimakon dTTP, kuma ana ƙara UNG enzyme wanda zai iya ƙasƙantar da samfur ɗin da ke ɗauke da dUTP a lokaci guda, wanda zai iya hana gurbatar samfuran haɓaka PCR.Ya ƙunshi gyare-gyare na musamman na D-Taq DNA Polymerase, dNTPs, MgCl2, buffer dauki, PCR dauki, ingantawa da stabilizer, da dai sauransu.2O zuwa madaidaicin 2× SuperEasyTMMix (UNG) -EDTA da za a yi amfani da shi a cikin amsawar PCR.
6× DNA Loading Buffer: Maɓallin Loading baya ƙunshi SDS.Ana ba da shawarar yin amfani da 6x DNA Loading Buffer wanda aka haɗa tare da kit lokacin yin aikin agarose gel electrophoresis don samun sakamako mai kyau na electrophoresis.Kada a yi amfani da Loading Buffer mai ɗauke da SDS, in ba haka ba za a sami babban wutsiya mai haske a cikin layi yayin electrophoresis, wanda zai shafi sakamakon gwaji.
Abubuwan Kit
| 2× SuperEasyTMMix(UNG) -EDTA |
| 6× DNA Loading Buffer |
| Umarni |
Fasaloli & fa'idodi
- Babu buƙatar tsarkakewar DNA mai cin lokaci da tsada, babu pretreatment, da amfani da jini kai tsaye azaman samfurin PCR.
-Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini.
-2× SuperEasyTMMix (UNG) - EDTA yana da babban jurewar jini: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
-Samfurin an rufe shi sosai, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.Tsarin PCR na rigakafin gurɓatawa 2 × SuperEasyTMMix (UNG) -EDTA, yadda ya kamata yana kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa, kuma yana tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Kit aikace-aikace
Ƙaddamarwa don kai tsaye ganewar PCR na EDTA wanda ke da kariya ga duka jini.(Cikin hada da: jinin mutum, jinin bera, jinin kaza, jinin tsuntsu, jinin saniya, jinin kare, da sauransu).
Gudun aiki
Adana da Rayuwar Shelf
Ana adana wannan kit ɗin a -20 ° C.
2× SuperEasyTMMix (UNG) -EDTA, Idan ana amfani dashi akai-akai, ana iya adana shi a 4°C don ajiyar ɗan gajeren lokaci (amfani cikin kwanaki 10).
6 × DNA Loading Buffer, Ana iya adana shi a 4°C ko -20°C na dogon lokaci.
