Blood SuperDirect yana PCR Kit-Heparin Jini Kai tsaye PCR Master Mix don Genotyping na Jini
Bayanin Kit:
Kai tsaye yi amfani da heparin anticoagulated jinin gabaɗayan azaman samfuri don haɓaka PCR, tare da babban hankali da ingantaccen haɓakawa.
◮Babu buƙatar tsarkakewar DNA mai cin lokaci da tsada, babu pretreatment, da amfani da jini kai tsaye azaman samfuri na PCR.
◮Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini a hankali.
◮2× SuperEasyTMHaƙuri-Heparin Haƙurin jini: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
◮Samfurin yana rufe cikakke, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.
Ƙayyadaddun bayanai
200 × 20μl rxns, 2000 × 20μl rxns
2× SuperEasyTMMix-Heparin: Ya ƙunshi musamman modified D-Taq DNA Polymerase, dNTPs, MgCl2, dauki buffer, PCR dauki enhancer, ingantawa, stabilizer, da dai sauransu A cikin PCR dauki, kawai ƙara dace anticoagulated dukan jini, firamare da ddH2O zuwa madaidaicin 2× SuperEasyTMMix-Heparin da za a yi amfani da shi a cikin amsawar PCR.
6× DNA Loading Buffer: Maɓallin Loading baya ƙunshi SDS.Ana ba da shawarar yin amfani da 6x DNA Loading Buffer wanda aka haɗa tare da kit lokacin yin aikin agarose gel electrophoresis don samun sakamako mai kyau na electrophoresis.Kada a yi amfani da Loading Buffer mai ɗauke da SDS, in ba haka ba za a sami babban wutsiya mai haske a cikin layi yayin electrophoresis, wanda zai shafi sakamakon gwaji.
Abubuwan Kit
| 2× SuperEasyTM Mix-Heparin |
| 6× DNA Loading Buffer |
| Umarni |
Fasaloli & fa'idodi
-Babu buƙatar tsarkakewar DNA mai cin lokaci da tsada, babu pretreatment, da amfani da jini kai tsaye azaman samfuri na PCR.
-Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini.
-2× SuperEasyTMHaƙuri-Heparin Haƙurin jini: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
-Samfurin an rufe shi sosai, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.
Kit aikace-aikace
An sadaukar don kai tsaye tantance PCR na Heparin anti-coagulated jini duka.(Cikin hada da: jinin mutum, jinin bera, jinin kaza, jinin tsuntsu, jinin saniya, jinin kare, da sauransu).
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana wannan kit ɗin a -20 ℃.
2× SuperEasyTMMix-Heparin, Idan ana amfani dashi akai-akai, ana iya adana shi a 4 ° C don ajiyar ɗan gajeren lokaci (amfani da sama a cikin kwanaki 10).
6 × DNA Loading Buffer, Ana iya adana shi a 4°C ko -20°C na dogon lokaci.
