Kit ɗin Cirar Gel na DNA Gel
Bayanin Kit:
Da sauri da kuma yadda ya kamata mai da 20bp-10kb DNA gutsuttsura daga agarose gel.
◮Faɗin farfadowa na DNA:Ana iya dawo da gutsuttsuran DNA gajarta kamar 30bp kuma girman 10kb.
◮Babban ingancin farfadowa:Mafi girman ingancin farfadowa zai iya kaiwa fiye da 80%.
◮Karamin tsarin haske:aƙalla 30μl bayani na elution za a iya amfani da shi don elution, wanda zai iya ƙara yawan ƙwayar DNA da aka gano.
◮Gudun sauri:Sauƙi don aiki, ana iya kammala dawo da guntun DNA a cikin mintuna 15.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:Rubutun DNA da aka dawo dasu suna da tsafta, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
Bayani
Wannan kit ɗin yana amfani da ginshiƙin juzu'i da dabarar da kamfaninmu ya haɓaka, wanda zai iya dawo da tsaftataccen gutsuttsarin DNA daga gel na agarose yadda ya kamata.Kit ɗin na iya dawo da gutsuwar DNA a cikin kewayo mai faɗi.A ƙarƙashin yanayi na al'ada, ana iya dawo da gutsuwar DNA na 30bp-10kb.Ana iya amfani da mafi ƙarancin 30μl na eluent don ƙara yawan tattara DNA da aka dawo dasu.
Kit ɗin yana da sauƙin aiki kuma yana da ƴan matakai.Yana buƙatar centrifugation da yawa kawai don aiwatar da samfurori da yawa a lokaci guda, kuma za'a iya samun tsattsauran tsaftar DNA da aka gano a cikin mintuna 15.
Ƙayyadaddun bayanai
50 Shirye-shirye
Buffer GE: Narkar da gel block a 60 ° C don saki DNA guntu daga gel.
Buffer WB1: Cire ragowar ions gishiri a cikin DNA.
Buffer EB: haɓaka DNA akan membrane ginshiƙin tsarkakewa.
Rukunin DNA-kawai: Takamaiman adsorption na guntun DNA a cikin gel.
Abubuwan Kit
| Buffer GE* |
| Farashin WB1 |
| Buffer EB |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Mai fa'ida na dawo da DNA: guntun DNA gajere kamar 30bp kuma girman 10kb ana iya dawo dasu.
-High farfadowa da na'ura mai aiki da karfin ruwa: Mafi girman yadda ya dace zai iya kaiwa fiye da 80%.
-Small tsarin elution: a kalla 30μl elution bayani za a iya amfani da elution, wanda zai iya yadda ya kamata ƙara maida hankali na DNA gutsuttsura.
-Mai saurin sauri: Sauƙi don aiki, ana iya kammala dawo da gutsuttsura DNA a cikin mintuna 15.
-Safety: Ba a buƙatar hakar reagent na halitta.
-Maɗaukaki mai inganci: Gutsun DNA da aka dawo dasu suna da tsafta, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
Kit aikace-aikace
Wannan kit ɗin ya dace don dawo da gutsuttsuran DNA (30bp-10kb) a cikin gels agarose.
Gudun aiki
Adana da Rayuwar Shelf
Ana iya adana wannan kit ɗin na tsawon watanni 12 a ƙarƙashin busassun yanayin zafi a ɗaki (15-25 ° C), idan ana buƙatar adana shi na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
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