Na'urar Keɓewar DNA ta Ruwa na DNA Ciro da Kayan Tsafta don Ruwa
Bayanin Kit:
Da sauri tsarkake high quality-genomic DNA daga microorganisms a cikin daban-daban samfurin ruwa.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Ƙayyadaddun bayanai
50 Shirye-shirye
- Lysozyme: enzymatically hydrolyze bango cell na tabbatacce kwayoyin.
-Buffer TE: amfani da shi don shirya 100mg / ml Lysozyme bayani da kuma samar da lysozyme enzymatic hydrolysis yanayi.
-Buffer SG1 & Buffer SG2: Samar da samfurin protease yanayin narkewa.
-Foregene Protease: Enzymatically hydrolyze samfurori a cikin yanayin enzymatic protease don saki DNA na genomic.
-Buffer SG3: Inactivate Foregene Protease da samar da yanayin lodin DNA.
-Buffer SG4: Kari don samar da mahallin lodin DNA.
-Buffer PW: Cire datti kamar furotin da RNA a cikin DNA.
-Buffer WB: Cire ragowar ions gishiri a cikin DNA.
-Buffer EB: haɓaka DNA akan membrane shafi na tsarkakewa.
-Shafin DNA-kawai: musamman adsorb DNA na genomic a cikin lysate.
Abubuwan Kit
| Farashin SG1 |
| Farashin SG2 |
| Farashin SG3 |
| Farashin SG4 |
| Farashin PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Maganin Protease |
| Lysozyme |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA na kwayar halitta ba tare da ƙarin RNase ba yayin gwajin, yana guje wa gurɓatar da dakin gwaje-gwaje ta RNase na waje.
-Saurin sauri: Aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na ruwa a cikin mintuna 40.
-Mafi dacewa: Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar 4 ° C ƙananan zafin jiki ko hazo na ethanol na DNA.
-Safety: babu bukatar Organic reagent hakar.
-Maɗaukakiyar inganci: Gaɓar halittar DNA da aka fitar suna da girma, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da tsarkakewa na DNA na kwayoyin halitta na samfurori masu zuwa: ruwa na ruwa na ruwa, ruwan kandami, ruwan famfo, ruwan tafkin, ruwan kogi, ruwan tafkin lotus da sauran samfurori.
Gudun aiki
Adana da Rayuwar Shelf
-Za'a iya ajiye wannan kayan na tsawon watanni 12 a karkashin busasshen yanayi a cikin daki (15-25°C), idan ana bukatar a adana shi na tsawon lokaci, za'a iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da saka maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheat shi a cikin 37°C ruwan wanka na tsawon mintuna 10 don narkar da hazo, sannan a gauraya shi kafin amfani.
-Foregene Protease bayani yana da tsari na musamman, wanda ke aiki lokacin da aka adana shi a dakin da zafin jiki na dogon lokaci (watanni 3);idan an adana shi a 4 ℃, aikinsa da kwanciyar hankali zai fi kyau, don haka ana bada shawara don adana shi a 4 ℃, ku tuna kada ku adana shi a -20 ℃.
-An adana busassun foda Lysozyme a -20 ° C;An raba maganin Lysozyme da aka shirya zuwa ƙananan ƙananan kuma an adana shi a -20 ° C.
