Tsarin Shuka (Babban) Kit ɗin PCR Kai tsaye II-UNG(ba tare da Kayan Aikin Samfura ba)
Bayanin Kit:
Yin amfani da lysate iri kai tsaye azaman samfuri don haɓakawa na PCR, babu warewar DNA daban, sauri da hankali mai girma.An kara tsarin hana gurbatar yanayi na UNG don hana abubuwan da ba su dace ba.
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.
◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.
Bayani
Wannan samfurin yana amfani da tsarin amsawa na musamman na lysis, wanda zai iya saki DNA da sauri daga samfurori iri iri tare da ƙananan polysaccharide da abun ciki na polyphenol (kamar: shinkafa, alkama, taba, masara, waken soya, da dai sauransu) don amfani a cikin halayen PCR, don haka ya dace musamman Manyan gwajin kwayoyin halitta.Domin saduwa da buƙatun gwaje-gwajen ganowa daban-daban, wannan kit ɗin na iya amfani da nau'ikan samfura da yawa (kamar dukan iri, ƙananan yankan nama, ko samfuran ƙasa na iri da yawa) don gwaje-gwaje.Daga cikin su, don samfuran iri waɗanda har yanzu suna buƙatar germination, zaku iya zaɓar yanke ƙananan ƙwayoyin cuta (1-5mg) ban da ƙwanƙwaran iri don gwaji;don gwaje-gwajen da ake buƙatar samfurin a cikin adadi mai yawa, za ku iya zaɓar don haɗa samfurori da yawa kuma ku haɗa su.Bayan niƙa cikin barbashi tare da diamita na kusan 0.5mm, ana iya yin gwajin (aƙalla 0.1% na samfurin da aka yi niyya ana iya gano shi).
Ƙayyadaddun bayanai
50T,200T,500T,2000T
Abubuwan Kit
| Kayan Shuka Kai tsaye PCR Kit II-UNG Irin Shuka (Babban) Kit-UNG PCR Kai tsaye | |||||
| Abun Haɗin Kit | Saukewa: TP-0314T | Saukewa: TP-03141 | Saukewa: TP-03142 | Saukewa: TP-03143 | |
| 50 T | 200T | 500T | 2000T | ||
| Kashi na I | Farashin SP1 | ml 30 | 120 ml | 100ml × 3 | 400ml × 3 |
| Farashin SP2 | 3ml ku | ml 10 | ml 25 | 100 ml | |
| 6× DNA Loading Buffer | 1.5ml ku | 1.5ml ku | 1.5ml ku | 1.5ml × 4 | |
| Kashi na II | 2× Seed PCR EasyTMMix(UNG) | 500 μl | 1 ml ×2 | 1.7ml × 3 | 1.7ml × 12 |
| Manual | 1 | 1 | 1 | 1 | |
Features da abũbuwan amfãni
- Babu buƙatar ɗaukar lokaci kuma ana buƙatar tsarkakewar DNA mai tsada.
- Buƙatun samfurin ƙananan ne, kawai ɗauki iri ɗaya.
- Ba a buƙatar jiyya na musamman kamar niƙa da murƙushewa, kuma aikin yana da sauƙi.
- Ingantaccen tsarin PCR yana bawa PCR damar samun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙaƙƙarfan juriya ga masu hana PCR.
- Tsarin PCR na rigakafin gurɓataccen abu 2 × Seed PCR EasyTMMix (UNG) na iya kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa da tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Gudun aiki
Yanayin ajiya
1.Tafi
Dukkanin tsarin sufurin akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin Sashe na II yana cikin yanayin <4 ℃.
2.Ajiye
Sashi na I na wannan kit ɗin yakamata a adana shi a zazzabi na ɗaki ko 2-8 ° C.
Ya kamata a adana sashi na II a -20 ° C.
