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Lnc-RT Heroᵀᴹ I (Tare da gDNase) (Super Premix don haɗin cDNA na farko daga lncRNA)
Super Premix don haɗin cDNA na farko daga lncRNA
Farashin RTL-09098/09099
Tsarin juzu'i mai sauri kuma mai matuƙar kulawa don ƙirƙirar cDNA na farko daga lncRNA.
Cire gDNA a cikin mintuna 2.
Minti 15 don kammala haɗin haɗin cDNA na farko
Tsarin jujjuya juzu'i mai girma
High thermal kwanciyar hankali, da mafi kyau duka dauki zafin jiki ne 42 ℃, kuma shi har yanzu yana da kyau reverse kwafin yi a 50 ℃.
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RT Easyᵀᴹ I Master Premix don haɗakarwar cDNA na farko
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 25 ne kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃,kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 45%, gishiri guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
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RT Easy II (tare da gDNase) Master Premix don haɗin cDNA na farko don PCR na ainihi tare da gDNase
-Ingantacciyar ikon cire gDNA, wanda zai iya cire gDNA a cikin samfuri a cikin mintuna 2.
-Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 15 kawai don kammala haɗin layin farko na cDNA.
-Complex samfuri: samfura tare da babban abun ciki na GC da hadaddun tsarin sakandare kuma ana iya juyawa tare da ingantaccen inganci.
-Tsarin jujjuya juzu'i mai girma, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
-Tsarin rubutun baya yana da babban kwanciyar hankali na thermal, mafi kyawun zafin jiki shine 42℃, kuma har yanzu yana da kyakkyawan aikin juzu'i a 50℃.
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RT Easyᵀᴹ II Master Premix don haɗawar cDNA na farko don PCR na ainihi
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 20 kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da babban kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃, kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 60%, gishirin guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
- 2× RT KO-SaukiTM Mix ya ƙara ingantattun abubuwan rubutu na baya (Random Primer, Oligo(dT)18 Farko).
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