Kifi na Zebra Kai tsaye PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
Bayanin Kit:
Yin amfani da lysate na zebrafish kai tsaye azaman samfuri don haɓakawa na PCR, babu warewar DNA daban-daban, sauri da hankali mai girma.An ƙara tsarin hana gurɓacewar yanayi na UNG don kawar da abubuwan da ba su dace ba.
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.
◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.
Bayani
Wannan kit ɗin yana amfani da tsarin buffer na musamman don sakin DNA da sauri daga zebrafish da sauran kyallen kifin ruwa mai kyau, wutsiya ko samfuran ƙwai don halayen PCR, don haka ya dace musamman don gwajin ƙwayoyin cuta masu girma.An kammala aiwatar da sakin kwayoyin halittar DNA daga ma'aunin lysis a cikin mintuna 10-30 a 65°C.Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma za a iya amfani da DNA ɗin da aka fitar azaman samfuri don amsawar PCR.
2× PCR EasyTM Mix (UNG) yana amfani da dUTP maimakon dTTP bisa tushen 2× PCR EasyTM Mix, kuma yana ƙara UNG enzyme (Uracil-N-glycosylase) wanda zai iya lalata samfurin da ke dauke da dUTP a lokaci guda.Kafin amsawar PCR, ana amfani da enzyme na UNG don lalata samfurin PCR mai ɗauke da uracil.UNG enzyme ba zai yi wani tasiri a kan samfuri wanda bai ƙunshi uracil ba, don haka tabbatar da ƙayyadaddun ƙayyadaddun ƙayyadaddun haɓakawa da kuma hana yiwuwar gwaje-gwaje masu girma na kwayoyin halitta.An sami gurɓata samfuran PCR.
D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
| Kashi na I | Buffer FP |
| Maganin Protease | |
| 6× DNA Loading Buffer | |
| Kashi na II | 2 × PCR Mai SauƙiTMMix(UNG) |
| Umarni | |
Fasaloli & fa'idodi
■ Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
∎ Bukatar samfurin ba ta da yawa, kamar yadda za a iya gwada fin caudal kifi 1 MG ko ƙwan kifi 10.
■ Ba a buƙatar magani na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
∎ Tsarin PCR Anti-Pollution 2× PCR EasyTM Mix (UNG), wanda zai iya kawar da gurɓacewar da samfuran PCR ke haifarwa yadda ya kamata da tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.
Kayan aiki sigogi
Aikace-aikace: Kifin Zebra da sauran kifin ruwa mai daɗi.
DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.
Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana sashe na I na wannan kit ɗin a 2-8 ℃.
Za'a iya adana buffer FP na reagent na watanni 12 a ƙarƙashin yanayin bushe;Ana iya adana shi a 2-8 ℃ don adana tsawon lokaci.
v Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aiki da kwanciyar hankali, da fatan za a adana shi a 4 ℃ na watanni 12.
vReagent 6×DNA Loading Buffer za a iya adana a 4 ℃ ko -20 ℃ na dogon lokaci.
Ya kamata a adana Sashe na II na wannan kit a -20 ℃.
vReagent 2×PCR EasyTM Mix(UNG), idan ana amfani dashi akai-akai, kuma ana iya adana shi a 4℃ don ajiyar ɗan gajeren lokaci (amfani sama da kwanaki 10).
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