Kifi na Zebra Kai tsaye PCR Kit Direct PCR Lysis Reagent(zebrafish)
Bayanin Kit:
Yi amfani da lysate na zebrafish kai tsaye azaman samfuri don haɓakawa na PCR, ba tare da ware DNA daban ba, sauri da hankali mai girma.
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.
◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.
Bayani
Wannan samfurin yana amfani da tsarin buffer na musamman na lysis don sakin DNA da sauri daga kifin zebrafish da sauran kyallen kifin ruwa mai daɗi, wutsiya ko samfuran ƙwai don halayen PCR, don haka ya dace da babban gwajin kwayoyin halitta.An kammala aiwatar da sakin kwayoyin halittar DNA daga ma'aunin lysis a cikin mintuna 10-30 a 65°C.Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma za a iya amfani da DNA ɗin da aka fitar azaman samfuri don amsawar PCR.
2 × PCR Mai SauƙiTMMix yana da ƙarfi mai ƙarfi ga masu hanawa na PCR, kuma yana iya amfani da lysate na samfurin don gwadawa azaman samfuri don ingantaccen haɓakawa da takamaiman.Wannan reagen ya ƙunshi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, amsawa buffer, PCR ingantawa da stabilizer.An yi amfani da shi tare da buffer lysis zai iya gano samfurori da sauri da sauƙi, kuma yana da halaye na babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi, da kwanciyar hankali mai kyau.
D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
| Kashi na I | Buffer FP |
| Maganin Protease | |
| 6× DNA Loading Buffer | |
| Kashi na II | 2 × PCR Mai SauƙiTMMix |
| Umarni | |
Fasaloli & fa'idodi
■Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
∎ Bukatar samfurin ba ta da yawa, kamar yadda za a iya gwada fin caudal kifi 1 MG ko ƙwan kifi 10.
■ Ba a buƙatar magani na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.
∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.
Kayan aiki sigogi
Aikace-aikace: Kifin Zebra da sauran kifin ruwa mai daɗi.
DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.
Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana sashe na I na wannan kit ɗin a 2-8 ℃.
Za'a iya adana buffer FP na reagent na watanni 12 a ƙarƙashin yanayin bushe;Ana iya adana shi a 2-8 ℃ don adana tsawon lokaci.
v Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aiki da kwanciyar hankali, da fatan za a adana shi a 4 ℃ na watanni 12.
vReagent 6×DNA Loading Buffer za a iya adana a 4 ℃ ko -20 ℃ na dogon lokaci.
Ya kamata a adana Sashe na II na wannan kit a -20 ℃.
vReagent 2×PCR EasyTM Mix, idan ana amfani dashi akai-akai, kuma ana iya adana shi a 4℃ don ajiyar ɗan gajeren lokaci (amfani cikin kwanaki 10).
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