ForeSNP Genotyping Kit
Bayanin Kit:
Babban daidaito: buga ingantaccen iko, ƙimar daidaito ya wuce 98%.
Ƙananan farashi: Babu buƙatar haɗa adadi mai yawa na bincike mai lakabi biyu masu tsada.
Ingantaccen tsarin PCR: kyakkyawan tsarin kwanciyar hankali da ƙayyadaddun haɓakawa mai ƙarfi.
Tsarin PCR Anti-Pollution: Yana iya kawar da gurɓataccen gurɓataccen iska da samfuran PCR ke haifarwa, ba tare da damuwa game da tsangwama na gurɓataccen muhalli akan siginar kyawu na kulawa mara kyau ba, da tabbatar da ƙayyadaddun haɓakawa da daidaiton bugawa.
Ƙayyadaddun bayanai
Fasahar PCR Specific Allele (Competitive Allele Specific PCR) sabuwar nau'in hanyar buga allele ce.Wannan hanyar ba ta buƙatar haɗa takamaiman bincike don kowane SNP da inDel, amma kawai yana buƙatar nau'i biyu na musamman na duniya don cimma daidaitaccen buga samfuran DNA na genomic.Ta hanyar nazarin ƙarfi da rabon siginar kyalli na ƙarshe, ana ƙayyade genotype ta atomatik, kuma tasirin tari yana nunawa a gani.Wannan hanya tana da ɗan gajeren lokacin ganowa, ƙananan farashin reagent, babban ganowa daidai, kuma ana iya amfani da shi don taimakon kiwo mai alamar ƙwayoyin cuta, saka QTL, gano alamun kwayoyin halitta da sauran gwaje-gwajen ilimin halitta na ƙwayoyin cuta tare da babban samfurin girma.
Ƙayyadaddun bayanai
5ml, 50ml, 50ml × 10, 500ml × 20
Abubuwan Kit
| 2× GT SaukiTMMix |
| ddH2O |
| Umarni |
Fasaloli & fa'idodi
■Babban daidaito: buga ingantaccen iko, ƙimar daidaito ya wuce 98%.
∎ Ƙananan farashi: Babu buƙatar haɗa ɗimbin ɗimbin bincike masu lakabi biyu masu tsada.
∎ Ingantaccen tsarin PCR: kyakkyawan tsarin kwanciyar hankali da ƙayyadaddun haɓakawa mai ƙarfi.
∎ Tsarin PCR Anti-Pollution: Yana iya kawar da gurɓataccen iska da samfuran PCR ke haifarwa, ba tare da damuwa game da tsoma bakin gurɓataccen muhalli ba akan siginar kyamarorin da ba a iya sarrafawa ba, da tabbatar da ƙayyadaddun haɓakawa da daidaiton bugawa.
Kayan aiki
An yi niyyar amfani da shi wajen gwajin ƙwayoyin halittar DNA da aka tsarkake.
Misali
A cikin wannan gwaji, an yi amfani da DNA na alkama 200ng azaman samfuri don gano nau'in halittar TaGS-D1 da ke da alaƙa da nauyin hatsin alkama a ƙarƙashin Foresnp Genotyping Kit.Samfurin kayan aiki shine haɗin BIO-RAD CFX;Yawan samfurori shine 21, adadin NTCs shine 8, kuma kowane nau'in sarrafawa mai kyau shine 1.
ForeSNP Genotyping Kit
