China Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(don Genotyping) Manufacturer and Supplier |Foregene

Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Mouse Tail) (don Genotyping)

Bayanin Kit:

Ana amfani da lysate wutsiyar linzamin kwamfuta kai tsaye azaman samfuri don haɓaka PCR ba tare da cire DNA daban ba, wanda yake da sauri da kulawa sosai.An ƙara tsarin hana gurɓacewar yanayi na UNG don kawar da abubuwan da ba su dace ba.

◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.

◮Bukatar samfurin ƙarami ne, ɗauki iri ɗaya kawai.

◮Ba a buƙatar jiyya na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.

◮Ingantacciyar tsarin PCR yana bawa PCR damar samun takamaiman takamaiman aiki da haƙuri mai ƙarfi ga masu hana PCR.

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Bayani

Wannan kit ɗin yana amfani da tsarin buffer na lysis na musamman don sakin DNA da sauri daga wutsiyar linzamin kwamfuta, kunnuwa, tsokoki da sauran samfuran nama don halayen PCR, don haka ya dace musamman don gwajin ƙwayar cuta mai girma.

An kammala aiwatar da sakin kwayoyin halittar DNA daga ma'aunin lysis a cikin mintuna 10-30 a 65°C.Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma za a iya amfani da DNA ɗin da aka fitar azaman samfuri don amsawar PCR.

2× M1-PCR Sauki^TMMix (UNG) yana amfani da dUTP maimakon dTTP akan 2× M1-PCR Easy^TMMix (UNG), kuma yana ƙara UNG enzyme (Uracil-N-glycosylase) wanda zai iya lalata samfurin da ke ɗauke da dUTP a lokaci guda.Kafin amsawar PCR, ana amfani da enzyme na UNG don lalata samfurin PCR mai ɗauke da uracil.UNG enzyme ba zai yi wani tasiri a kan samfuri wanda bai ƙunshi uracil ba, don haka tabbatar da ƙayyadaddun ƙayyadaddun ƙayyadaddun haɓakawa da kuma hana yiwuwar gwaje-gwaje masu girma na kwayoyin halitta.An sami gurɓata samfuran PCR.

D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.

Ƙayyadaddun bayanai

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Abubuwan Kit

Kashi na I	Buffer MP
	Foregene Protease Plus
	6× DNA Loading Buffer
Kashi na II	2 × M1 PCR Mai Sauƙi^TMMix(UNG)
Umarni

Fasaloli & fa'idodi

■Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.

∎ Bukatar samfurin karami ne, kamar yadda za'a iya gwada 5mg na linzamin kwamfuta ko wutsiya 2-5mm.

■ Ba a buƙatar magani na musamman kamar niƙa da murkushewa, kuma aikin yana da sauƙi.

∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.

2 Tsarin PCR Anti-Pollution×M1-PCR Sauki^TMMix (UNG), wanda ke kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa kuma yana tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.

Kayan aiki sigogi

Iyakar aikace-aikacen: wutsiya linzamin kwamfuta, kunnen linzamin kwamfuta, tsoka da sauran kyallen takarda.

DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.

Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.

Gudun aiki

Adana da Rayuwar Shelf

Ya kamata a adana sashe na I na wannan kit ɗin a 2-8 ℃.

Ana iya adana buffer AL reagent na watanni 12 a ƙarƙashin bushewa;Ana iya adana shi a 2-8 ℃ don adana tsawon lokaci.

Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aiki da kwanciyar hankali, da fatan za a adana shi a 4 ℃ na watanni 12.

Reagent 6 × DNA Loading Buffer za a iya adana shi a 4 ℃ ko -20 ℃ na dogon lokaci.

Ya kamata a adana Sashe na II na wannan kit a -20 ℃.

Reagent 2 × M1 PCR EasyTM Mix (UNG), idan ana amfani dashi akai-akai, ana iya adana shi a 4℃ don ajiyar ɗan gajeren lokaci (amfani sama da kwanaki 10).

Na baya: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent (Mouse Tail)(don Genotyping)

Na gaba: Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Mouse Tail) (don Genotyping)

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