Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent (Mouse Tail)(don Genotyping)
Ƙayyadaddun bayanai
Wannan kit ɗin yana amfani da tsarin buffer na lysis na musamman don sakin DNA da sauri daga wutsiyar linzamin kwamfuta da samfuran kunnen linzamin kwamfuta don amsawar PCR, don haka ya dace musamman don gwajin ƙwayoyin cuta masu girma.
An gama aiwatar da sakin DNA na genomic daga ma'aunin lysiscikin minti 10-30azafin jiki (20-25°C).Ba a buƙatar dumama, kuma ba a buƙatar wasu matakai kamar furotin da cire RNA.Ana iya amfani da adadin adadin DNA da aka fitar azaman Samfura don amsawar PCR.
2 × M-PCR Mai SauƙiTM Mix yana da ƙarfi mai ƙarfi ga masu hanawa na PCR, kuma yana iya amfani da lysate na samfurin don gwadawa azaman samfuri don ingantaccen haɓakawa da takamaiman.Wannan reagen ya ƙunshi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, amsawa buffer, PCR ingantawa da stabilizer.
D-Taq DNA polymerase shine DNA polymerase wanda Foregene ya haɓaka musamman don halayen PCR kai tsaye.D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'ikan hanawa na PCR, kuma yana iya haɓaka adadin DNA da kyau a cikin tsarin ɗaukar nauyi daban-daban, kuma saurin haɓakawa zai iya kaiwa 2Kb/min, wanda ya dace musamman don amsawar PCR kai tsaye.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
Kashi na I | Farashin NP |
Foregene Protease Plus I | |
6× DNA Loading Buffer | |
Kashi na II | 2× M PCR EasyTM Mix |
Umarni |
Fasaloli & fa'idodi
■Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
∎ Bukatar samfurin ƙarami ne, kawai ɗauki wutsiyar linzamin kwamfuta (ko kunnuwan linzamin kwamfuta tare da diamita na 2-5mm) sama da 2mm.
∎ Sauƙi don aiki, ana iya shafa shi ba tare da dumama don kunna protease ba, kuma samfurin da aka yi masa za a iya fuskantar da PCR kai tsaye.
■ Za a iya amfani da Kit ɗin Haɓakar DNA na Mouse Tail Genomic (DE-05211) don cire DNA daga wutsiyar bera da aka sarrafa.
∎ Ingantaccen tsarin PCR yana sa PCR su sami ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da ƙarfin juriya ga masu hana PCR dauki.
Kayan aiki sigogi
Aikace-aikace: wutsiya na linzamin kwamfuta, kunnuwa linzamin kwamfuta, tsoka da sauran kyallen takarda.
DNA da aka saki daga samfurin lysis: kawai ana amfani dashi azaman samfuri na PCR.
Ana iya amfani da kit ɗin don dalilai masu zuwa: ganewar transgenosis, genotyping dabba, da dai sauransu.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana sashe na I na wannan kit ɗin a 2-8 ℃.
Za'a iya adana buffer FP na reagent na watanni 12 a ƙarƙashin yanayin bushe;Ana iya adana shi a 2-8 ℃ don adana tsawon lokaci.
Reagent Foregene Protease yana da tsari na musamman.Don tabbatar da aiki da kwanciyar hankali, da fatan za a adana shi a 4 ℃ na watanni 12.
Reagent 6 × DNA Loading Buffer za a iya adana shi a 4 ℃ ko -20 ℃ na dogon lokaci.
Ya kamata a adana Sashe na II na wannan kit a -20 ℃.
Reagent 2×PCR EasyTM Mix, idan ana amfani dashi akai-akai, ana iya adana shi a 4 ℃ don ajiyar ɗan gajeren lokaci (amfani da sama a cikin kwanaki 10).