Na'urar Warewar DNA Bacterial Na'urar Tsabtace Tsabtace Bacterial Genomic DNA
Bayanin Kit:
Da sauri tsarkake DNA mai inganci mai inganci daga ƙwayoyin cuta a cikin lokacin haɓaka logarithmic.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayani
Wannan kit ɗin yana ba da hanya mai sauri da sauƙi don cire DNA na genomic daga al'adun ƙwayoyin cuta (Gram-negative da Gram-positive) daga tushe daban-daban;yana iya sarrafa ƙasa da 3ml na al'adun ƙwayoyin cuta a cikin lokacin haɓaka logarithmic (kwayoyin cuta 1 × 109).An daidaita kit ɗin tare da ingantaccen inganci na Foregene Protease, wanda ke ba da damar fitar da 15-50μg na DNA mai inganci mai inganci a cikin awa 1.Bugu da kari, kit din na iya fitar da kwayoyin halitta ban da kwayoyin halitta, irin su plasmids, Cosmid, BAC, da sauransu.
Kayan DNA-kawai silica gel matrix abu da aka yi amfani da shi a cikin ginshiƙi na juzu'in sabon abu ne na musamman na kamfanin, wanda zai iya dacewa da kuma musamman tallata DNA.Matsakaicin ƙarfin adsorption na DNA shine 80μg.Tsarin buffer na musamman da tsarin haɓakawa na iya haɓaka kawar da RNA, sunadaran najasa, ions da sauran mahadi na halitta a cikin sel.Rukunin DNA ɗin da aka fitar yana da girma, tsafta mai ƙarfi, tsayayye kuma ingantaccen inganci, kuma girman guntuwar DNA yana da ƙarfi a kusan 23kb.
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Farashin ML1 |
| Farashin ML2 |
| Farashin PW |
| Buffer WB |
| Buffer EB |
| Maganin Protease |
| Lysozyme |
| Buffer TE |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Babu buƙatar ƙara RNase don cire RNA a cikin DNA ɗin kwayoyin halitta, guje wa gurɓatar dakin gwaje-gwaje ta RNase na waje.
-Mai saurin sauri: Foregene Protease yana da ayyuka mafi girma fiye da nau'in proteases iri ɗaya kuma yana iya narkar da samfuran ƙwayoyin cuta a cikin ɗan gajeren lokaci.
-Daɗi: Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar 4 ° C ƙananan zafin jiki ko hazo na ethanol na DNA.
-Safety: babu Organic reagent hakar da ake bukata.
-Maɗaukakiyar inganci: Gaɓar halittar DNA da aka fitar suna da girma, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Wannan kit ɗin ya dace don tsarkake DNA na genomic samfurori masu zuwa: Gram-negative bacteria da Gram-positive bacteria a cikin logarithmic girma lokaci.
Gudun aiki
Adana da Rayuwar Shelf
-Wannan kit ɗin za a iya adana shi na tsawon watanni 12 a ƙarƙashin busassun yanayi a cikin zafin jiki (15-25 ° C);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da saka maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
-Foregene Protease bayani yana da tsari na musamman, wanda ke aiki lokacin da aka adana shi a dakin da zafin jiki na dogon lokaci (watanni 3);lokacin da aka adana shi a 4 ° C, aikinsa da kwanciyar hankali zai fi kyau, don haka ana bada shawara don adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20 ° C.
-Lysozyme busasshen foda ne, wanda ke aiki idan an adana shi a 2-8 ° C na dogon lokaci (watanni 3).Don dogon ajiya, da fatan za a adana shi a -20 ° C.
