RT-PCR Easyᵀᴹ II (Mataki Biyu)
Bayanin Kit:
◮Ana iya aiwatar da ƙididdigar ƙididdigewa na RNA cikin sauri da kuma daidai.
◮Kit ɗin yana amfani da na musamman na Foregene reverse reagent reagent da Foregene HotStar Taq DNA Polymerase haɗe tare da keɓaɓɓen tsarin amsawa don inganta ingantaccen haɓakawa da ƙayyadaddun halayen.
◮ Ingantattun tsarin amsawa yana sa abin ya sami mafi girman ganewar ganewa, ingantaccen yanayin zafi, da mafi kyawun haƙuri.
Ƙayyadaddun bayanai
RT-PCR Mai SauƙiTM II(Mataki Biyu) ya ƙunshi duk reagents don matakai biyu na RT-PCR, haɗawa da amsawar RT da amsawar PCR a cikin wannan kit ɗin, samar da ingantacciyar RT Mix da PCR Mix.RT Mix na musamman na iya haɗa cDNA mai inganci na farko cikin sauƙi da inganci. 2× PCRSauƙiTM MixƘari yana da babban inganci da ƙayyadaddun haɓakawa.Haɗin kwayoyin halitta na biyu yana sa gano kwayar halittar da aka yi niyya ta fi damuwa.
Abubuwan Kit
| RT-PCRSauƙiTM II (Mataki Biyu) | ||
| Abubuwan da ke ciki | RT-02021 | RT-02022 |
| 100T(20 mltsarin) | 500T(20 mltsarin) | |
| 2× RT SaukiTM Mix | 1ml | 1.7ml×3 |
| 2× PCRSauƙiTM MixƘari | 1ml | 1.7ml×3 |
| Random Primer (50μM) | 200μl | 1ml |
| Oligo(dT)18 Firamare (50μM) | 200μl | 1 ml |
| RNase-Free ddH2O | 1.7ml ku | 1.7ml ku |
| Jagoran Jagora | 1yanki | 1yanki |
Fasaloli & fa'idodi
-Ana iya aiwatar da ƙididdigar ƙididdigewa na RNA cikin sauri da kuma daidai.
-Kit ɗin yana amfani da na musamman na Foregene reverse reagent reagent da Foregene HotStar Taq DNA Polymerase haɗe tare da keɓaɓɓen tsarin amsawa don inganta ingantaccen haɓakawa da ƙayyadaddun halayen.
-Ingantattun tsarin amsawa yana sa abin ya sami mafi girman ganewar ganewa, ingantaccen yanayin zafi, da mafi kyawun haƙuri.
Gudun aiki
Adana da Rayuwar Shelf
1.Yanayin sufuri
Dukkanin tsarin sufurin akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin yana cikin yanayin <4 °C.
2. Yanayin ajiya
Ana adana kit ɗin a -20 ° C.Ajiye samfurin a cikin firiji mai yawan zafin jiki a -20°C nan da nan bayan an karɓa.Idan yanayin ajiya ya dace,tsamfurin ba zai lalata duk wani aiki ba yayin lokacin ingancin shekara 1.
