EndoFree Maxi Plasmid Kit (Spin Column)
Bayanin Kit:
Duk aikin hakar yana ɗaukar awa 1 kawai, yana tabbatar da dacewa da aiki mai sauri.
Bayani
Kit ɗin Plasmid na EndoFree Maxi yana ɗaukar dabarar tallan siliki ta musamman don ɗaure DNA plasmid da kyau da inganci.Ta haɗa na musamman na Endotoxin Removal Buffer P4 da EndoFree Maxi Filtration Columns, endotoxin, sunadarai da sauran ƙazanta ana iya cire su da kyau.Duk aikin hakar yana ɗaukar awa 1 kawai, yana tabbatar da dacewa da aiki mai sauri.
Adadin ƙwayoyin cuta da aka ba da shawarar ga kowane aikin: Don babban kwafin plasmid, ana ba da shawarar yin amfani da 100 ml na kafofin watsa labarai na al'ada na ƙwayoyin cuta, kuma yawan amfanin ƙasa gabaɗaya kusan 500-1500 μg.Don ƙananan kwafin plasmid, 200 ml kafofin watsa labarai na al'ada na ƙwayoyin cuta ana ba da shawarar samar da kusan 200-600 μg plasmids.
Abubuwan Kit
| Samfura Abubuwan da aka gyara | D909-E(10 preps) |
| Buffer BL | ml 30 |
| Buffer P1 | 100 ml |
| Buffer P2 | 100 ml |
| Buffer P4 | 100 ml |
| Farashin DW2 | ml 44 |
| Buffer EB | ml 30 |
| RNase A (10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 inji mai kwakwalwa |
| FinePure Maxi tace sirinji | 10 inji mai kwakwalwa |
| 50 ml Tarin Bututu | 10 inji mai kwakwalwa |
Gano Halin DNA na Plasmid da Tsafta
Ana iya gano tsabta da tattarawar DNA ɗin plasmid da aka fitar ta hanyar agarose gel electrophoresis da spectrophotometer ultraviolet.Ƙimar OD260 na 1 yayi daidai da kusan 50 μg/ml na DNA mai ɗaure biyu.Ƙimar OD260/OD280 na DNA plasmid da aka tsarkake gabaɗaya yana cikin kewayon 1.7-1.9.Ana iya amfani da DNA ɗin plasmid ɗin da aka tsarkake kai tsaye zuwa gwaje-gwaje tare da manyan buƙatun tsafta, kamar canjin tantanin halitta ko gwaje-gwajen vivo.
Kit aikace-aikace
DNA plasmid da aka fitar ta wannan kit ɗin za a iya amfani da shi zuwa ayyuka daban-daban na yau da kullun, gami da narkewar enzymatic, PCR, sequencing, ligation, canji da kuma canzawa ga nau'ikan tantanin halitta daban-daban.
Adana da Rayuwar Shelf
EndoFree Maxi Plasmid Kit za a iya adana shi a zazzabi na ɗaki (15-25°C) ƙarƙashin bushewar yanayi har zuwa watanni 12.Ƙara RNase A zuwa Buffer P1 kafin amfani na farko, gauraya da kyau kuma adana a 2-8°C.Za'a iya adana haɗe-haɗe a tsaye sama da watanni 6.
