Kits ɗin Keɓewar DNA na Stool Kayan Tsabtace DNA Daga Stool
Bayanin Kit:
Da sauri tsarkakewa da samun ingantaccen DNA na genomic daga samfuran stool iri-iri.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayani
Wannan kit ɗin yana ba da hanya mai sauri da sauƙi don fitar da DNA daga tushen ƙasa daban-daban.Akwai adadi mai yawa na masu hanawa kamar humic acid da ions ƙarfe a cikin samfuran ƙasa.Ko da waɗannan abubuwa sun kasance a cikin ƙananan kuɗi a cikin DNA mai tsabta, za su yi tasiri a kan halayen da ke ƙasa, kamar PCR da ƙuntataccen ƙwayar enzyme.Saboda haka, mabuɗin don tsarkake DNA na ƙasa shine yadda za a cire masu hanawa a cikin ƙasa yadda ya kamata.
Wannan kit ɗin yana amfani da DNA-Only Column wanda zai iya ɗaure musamman ga DNA, sabon-sabon Foregene Protease da kuma tsarin buffer na musamman, wanda zai iya cire masu hanawa daban-daban daga ƙasa yadda ya kamata ba tare da hakowar kwayoyin halitta ko ethanol da hazo na isopropanol ba.Ana iya kammala cire DNA daga samfuran ƙasa a cikin mintuna 90.
Ƙayyadaddun bayanai
Shirye-shirye 25, Shirye-shiryen 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Farashin SL1 |
| Farashin SL2 |
| Farashin SL3 |
| Farashin PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Maganin Protease |
| Lysozyme |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga gurɓata ta RNase na waje.
-Mai saurin sauri: Aikin yana da sauƙi, kuma ana iya kammala aikin cirewar DNA na fecal a cikin mintuna 40.
-Mafi dacewa: Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar 4 ° C ƙananan zafin jiki ko hazo na ethanol na DNA.
-Safety: Ba a buƙatar hakar reagent na halitta.
-Maɗaukakiyar inganci: DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da tsarkakewar DNA na kwayoyin halitta daga samfurori masu zuwa: sabo ko daskararre mutum, saniya, kaza, linzamin kwamfuta da sauran samfurori na fecal.
Gudun aiki
Adana da Rayuwar Shelf
-Wannan kit ɗin za a iya adana shi na tsawon watanni 12 a ƙarƙashin busassun yanayi a cikin zafin jiki (15-25 ° C);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
- Maganin Foregene Protease yana da nau'i na musamman, wanda ke aiki idan an adana shi a dakin da zafin jiki na tsawon lokaci (watanni 3), aikinsa da kwanciyar hankali zai fi kyau idan an adana shi a 4 ° C, don haka ana bada shawara a adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20 ° C.
-Dry Lysozyme foda an adana shi a -20 ° C, an raba maganin Lysozyme da aka shirya zuwa ƙananan ƙananan kuma an adana shi a -20 ° C.
