Kit ɗin keɓewar DNA mara-hannu
Bayani
DNA wanda ba shi da salula (cfDNA) guntu ne na cfDNA da aka fitar daga sel zuwa cikin jini wanda zai iya ɗaukar bayanan halitta dagaciwace-ciwace, pathogens da tayi.Binciken cfDNA yana ba da alamomi masu mahimmanci don ganewar asali, ganowa da kuma lura da ci gaban cututtukasion.A halin yanzu, an yi amfani da wannan fasaha sosai a cikin fagagen gano maye gurbi, jagorar magani da kuma hasashen hasashen,gano pathogen da kuma marasa cin zarafi prenatal nunawa.
Kit ɗin keɓewar DNA mai kyauta na Foregene an ƙirƙira shi don ware DNA mai kyauta ta tantanin halitta (cfDNA) daga jini, ruwan jini, ruwan jiki, ko samfuran fitsari.Kit ɗin na iya keɓe cfDNA cikin sauri da dacewa cikin matakai uku: lysis/ ɗaure, wankewa da elution.Yana amfani da ƙirar silica-mai rufi na musamman na superparamagnetic beads tare da keɓaɓɓen tsarin lysis/na ɗaure don dawo da ƙwayoyin nucleic da ke yawo ta hanyar haɗin gwiwar hydrogen da ƙarfin lantarki, ba tare da sunadaran sunadarai da sauran ƙazanta ba.Ya dace da manyan tashoshin hakar mai sarrafa kansa mai ƙarfi don shirye-shiryen kowane bincike na ƙasa ta amfani da PCR ko NGS.
Kayan abun ciki da Ma'ajiya
Tebura 1 Kit ɗin Keɓewar Halittar Halitta na Ƙaunar Halitta na DNA
Cabubuwa | Rm | Adadin(25 T) | Adadin(50 T) | Adadin(100 T) | Storage |
Akwati1 | Buffer AL | 42 mlx1 kwalban | 89 mlx1 kwalban | 189 mLx1 kwalban | 10-30°C |
Farashin AW1 | 38 mlx1 kwalban | 83 mlx1 kwalban | 165 mlx1 kwalban | 10-30°C | |
Farashin AW2 | 15 mlx1 kwalban | 33 mlx1 kwalban | 66 mlx1 kwalban | 10-30°C | |
Haske | 3 mlx1 kwalban | 6 mlx1 kwalban | 12 mlx1 kwalban | 10-30°C | |
Akwati2 | Magnetic Beads A | 3.2 mLx1 kwalban | 6.5 mlx1 kwalban | 13 mlx1 kwalban | 2-8 ° C |
Proteinase K | 5 mlx1 kwalban | 10.5 mlx1 kwalban | 21 mlx1 kwalban | 2-8 ° C |
Bayanan kula:
[1] Kada a haxa abubuwan da aka haɗa daga batches daban-daban na kayan aiki.
[2] Buffer AL na iya haifar da hazo, wanda baya shafar aikinsa.Idan ana ganin hazo, da fatan za a sanya kwalban reagent a cikin wanka na ruwa 56 ° C na 1 0 - 20 min har sai hazo ya narke.Sa'an nan kuma Mix sosai kafin amfani.
[3] Kar a daskare Gilashin Magnetic A.
Tsari
Fasaloli & fa'idodi
-Ingantacciyar Haka Babban haɓakar hakar DNA, tsabta mai kyau, ingantaccen kawar da ƙazanta da masu hana PCR na ƙasa;
M Samfuran Haɗin kaiSauƙaƙan cirewar cfDNA daga samfuran 0.5mL zuwa samfuran 4mL tare da ƙarar ƙira kamar ƙasa da 20uL
Aikace-aikace Daban-dabanAn tabbatar da shi ta samfuran asibiti, an tsara shi don ƙari, kamuwa da cuta da ganewar haihuwa tare da biopsy na ruwa
Faɗin tallafi na Platform Mai sarrafa kansa Mai jituwa tare da manual ans mai sarrafa kansa hanyoyin hakar, goyon bayan Tecan, Hamilton, PE da sauran nucleic acid hakar workstations.