Babban Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Bayanin Kit:
Da sauri tsarkake DNA na plasmid mai inganci daga ƙwayoyin cuta da suka canza don gwaje-gwajen nazarin halittu na yau da kullun kamar canji da narkewar enzyme.
◮Cire RNA ba tare da ƙara RNase ba
◮Dace- ana yin centrifugation a dakin da zafin jiki
◮Mai sauri- ana iya kammala aikin a cikin mintuna 20
◮Amintacciya- ba a yi amfani da reagent Organic ba
◮Babban tsarki— OD260/280≈1.7-1.9
Bayanin Kit
Za'a iya fitar da samfuran nucleic acid na FOREGENE da samfuran samfuran tsarkakewa zuwa manyan acid nucleic daga tushe iri-iri da samfurori masu rikitarwa.Jerin samfuran hakar DNA sun dogara ne akan ginshiƙan tsarkakewa na DNA-kawai silica membrane da reagents na musamman waɗanda zasu iya tsarkake DNA mai inganci daga samfura iri-iri.
Janar Plasmid Mini Kit yana amfani da fasahar ginshiƙin tsarkakewa-Kawai da ingantaccen tsarin SDS lysis don samun ingantaccen DNA na plasmid daga ƙwayoyin cuta a cikin mintuna 20.DNA-kawai silica gel membrane da aka yi amfani da shi a cikin kashin baya shine sabon abu na musamman na FOREGENE, wanda zai iya tallata DNA yadda ya kamata kuma musamman, kuma yana cire RNA, sunadarai marasa ƙazanta, ions da sauran mahaɗan kwayoyin halitta a cikin sel zuwa mafi girma.Matsakaicin iya aiki na plasmid DNA na DNA-kawai ginshiƙin tsarkakewa shine 60μg DNA.Adadin DNA plasmid a zahiri da aka fitar yana da alaƙa da ƙarar maganin ƙwayoyin cuta, adadin kwafin plasmid (babban kwafi, ƙaramin kwafi), damuwa, yanayin al'adu da sauran dalilai.
Ƙayyadaddun bayanai
| Babban Plasmid Mini Kit | |||
| Kit abun ciki | Farashin DE-01001 | Farashin 01002 | Farashin 01003 |
| 50T | 100T | 250T | |
| Buffer S1 | ml 10 | ml 20 | ml 50 |
| Buffer S2 | ml 10 | ml 20 | ml 50 |
| Buffer S3 | ml 25 | ml 50 | ml 125 |
| Farashin PW | ml 25 | ml 50 | ml 125 |
| Farashin WB2 | ml 15 | ml 30 | ml 75 |
| Buffer EB | ml 10 | ml 20 | ml 50 |
| Rukunin DNA-Kawai | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA a cikin DNA ɗin plasmid ba tare da ƙara RNase ba yayin gwajin, guje wa gurɓatar dakin gwaje-gwaje ta hanyar RNase na waje.
- Saurin sauri: Ana iya kammala hakar DNA na Plasmid a cikin mintuna 20.
- Amintacce: Ba a buƙatar hakar reagents na halitta.
- Babban inganci: plasmid da aka samu ta hanyar cirewa ba shi da RNA, babu RNase da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Cirar DNA na Plasmid da tsarkakewa don ƙwayoyin cuta na Gram-korau.
Gudun aiki
zane
Adana da rayuwar shiryayye
Ana iya adana wannan kit ɗin na tsawon watanni 12 a ƙarƙashin yanayin bushewa a cikin zafin jiki (15-25 ℃);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a cikin 2-8 ℃.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheat shi a cikin wanka na ruwa 37 ℃ na tsawon minti 10 don narkar da hazo, sannan a gauraya shi kafin amfani.
