Kit ɗin Midi na DNA na Jini (1-5ml) Keɓewar Midi Kits don DNA daga Jini
Bayanin Kit:
Da sauri tsarkake babban ingancin genomic DNA daga jinin anticoagulated (1-5ml).
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayani
Samfurin jinin ya ƙunshi adadi mai yawa na jajayen ƙwayoyin jini da ƙwayoyin platelet, wanda ke sa sarrafa jini ya fi damuwa.Duk da haka, kwayar halittar jini ta DNA da aka fitar ta hanyar gabaɗaya ba ta da girma cikin tsabta kuma abun ciki ya yi ƙasa sosai.Wannan kit ɗin yana amfani da sabon-sabon Foregene Protease Plus da keɓaɓɓen tsarin BL1 da BL2, wanda zai iya narkar da jini gaba ɗaya.coagulationsamfurori a cikin mafi ƙanƙan lokaci, don haka guje wa lalata DNA zuwa mafi girma da samun mafi girman adadin DNA na genomic.Tsarin sarrafa jini mai sauri da aiki mai sauƙi na juzu'in juzu'i yana sauƙaƙa sosai da hako kwayoyin halittar jini, ta yadda za'a iya samun inganci mai inganci, tsaftataccen DNA a cikin mintuna 40.
DNA-kawai silica gel membrane da aka yi amfani da shi a cikin kashin ginshiƙi na wannan kit ɗin wani sabon abu ne na musamman na kamfanin, wanda ya dace kuma yana tallata DNA na musamman, kuma yana iya cire RNA, sunadarai marasa ƙazanta, ions da sauran mahadi na halitta a cikin sel zuwa mafi girma.Rukunin DNA da aka samu suna da girma, tare da tsafta mai ƙarfi, tsayayye da ingantaccen inganci, kuma matsakaicin ɗaukar nauyin ginshiƙi shine 80 μg DNA.Ana iya amfani da DNA ɗin da aka samu don gwaje-gwajen ilimin halitta kamar ƙuntatawa narkewa, PCR, haɗakar kudanci, ginin ɗakin karatu da sauransu.Kit ɗin na iya sarrafa har zuwa 5ml na jini a lokaci ɗaya don samun isassun adadin DNA mai tsabta.
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Farashin BL1 |
| Buffer BL2* |
| Buffer DC |
| Buffer PW* |
| Farashin WB1 |
| Buffer EB |
| Foregene Protease Plus |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA na kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana guje wa gurɓatar da dakin gwaje-gwaje ta RNase na waje.
-Mai saurin sauri: Foregene Protease Plus yana da ayyuka mafi girma fiye da nau'ikan proteases iri ɗaya, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 40.
-Mafi dacewa: Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar 4°C low-zazzabi centrifugation ko ethanol hazo na DNA.
-Safety: Ba a buƙatar hakar reagent na halitta.
-Maɗaukakiyar inganci: DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da cirewa da tsarkakewa na DNA na genomic daga sabo ko daskararre anticoagulated jini duka.
Gudun aiki
Adana da Rayuwar Shelf
Za'a iya adana wannan kit ɗin na tsawon watanni 12 a ƙarƙashin busassun yanayi a zazzabi na ɗaki (15-25℃);idan yana buƙatar adana na dogon lokaci, ana iya adana shi a cikin 2-8℃.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
-Foregene Protease Plus bayani yana da nau'i na musamman, wanda ke aiki idan an adana shi a dakin da zafin jiki na tsawon lokaci (watanni 3), aikinsa da kwanciyar hankali zai fi kyau idan an adana shi a 4 ° C, don haka ana ba da shawarar adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20.℃.
