FFPE DNA keɓe Kit ɗin Tsabtace DNA daga Samfuran FFPE
Bayanin Kit:
Da sauri tsarkake DNA mai inganci mai inganci daga kyallen da aka haɗa da paraffin kamar sassan paraffin da tubalan paraffin.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayani
An sadaukar da wannan kit ɗin don cirewa da kuma tsarkake DNA mai inganci mai inganci daga nama mai kayyade formaldehyde ko nama mai sanya paraffin.
DNA na genomic na samfurin da aka saka paraffin zai zama lalacewa saboda lokacin ajiya ko yanayi, yana da wuya a cire DNA na kwayoyin halitta.Wannan kit ɗin yana ɗaukar ingantacciyar dabara ta musamman, wacce ta dace da zazzage DNA na genomic daga samfuran da aka haɗa da paraffin tare da babban nasarar hakar.Dukan tsari baya buƙatar amfani da phenol mai guba, chloroform ko hazo isopropanol.
Wannan kit ɗin yana amfani da ginshiƙin juzu'i wanda zai iya ɗaure musamman DNA, sabon-sabon Foregene Protease Plus, da kuma wani tsarin buffer na musamman, wanda zai iya fitar da DNA na nama mai inganci mai inganci a cikin sa'o'i 2-5.
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Farashin FL1 |
| Buffer FL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA na kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana guje wa gurɓatar da dakin gwaje-gwaje ta RNase na waje.
-Mai saurin sauri: Foregene Protease Plus yana da ayyuka mafi girma fiye da nau'ikan proteases iri ɗaya, kuma yana narkar da samfuran nama cikin sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin sa'o'i 2-5.
-Daɗi: Ana yin centrifugation a cikin zafin jiki, kuma babu buƙatar 4°C low-zazzabi centrifugation ko ethanol hazo na DNA.
-Safety: babu Organic reagent hakar da ake bukata.
-Maɗaukakiyar inganci: Gaɓar halittar DNA da aka fitar suna da girma, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da cirewa da tsarkakewa na DNA na genomic daga samfurori na formaldehyde-kafaffen samfurori da samfurori na nama na paraffin.
Gudun aiki
Adana da Rayuwar Shelf
-Wannan kit ɗin za a iya adana shi na tsawon watanni 12 a ƙarƙashin busassun yanayi a cikin zafin jiki (15-25 ° C);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da saka maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
-Foregene Protease Plus bayani yana da tsari na musamman wanda ke aiki lokacin da aka adana shi a cikin zafin jiki na dogon lokaci (watanni 3);ya fi aiki da kwanciyar hankali lokacin da aka adana shi a 4 ° C, don haka ana bada shawarar adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20 ° C.
