Mini Kit na jini DNA
Bayanin Kit:
Da sauri tsarkake babban ingancin genomic DNA daga jinin da ba a rufe ba (<1ml).
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayanin Kit
Wannan kit ɗin yana ɗaukar sabon Foregene Protease Plus da na musamman na BL1 da BL2 tsarin buffer, wanda zai iya narkar da samfuran jini gaba ɗaya a cikin ɗan gajeren lokaci, don haka guje wa lalata DNA zuwa matsakaicin digiri da samun matsakaicin adadin DNA na genomic.Tsarin sarrafa jini mai sauri da kuma aiki mai sauƙi na DNA-Only yana sauƙaƙa sosai da hako kwayoyin halittar jini, yana sa DNA ɗin za a iya samun inganci mai inganci da tsafta a cikin mintuna 40.
DNA-kawai silica gel membrane da aka yi amfani da shi a cikin ginshiƙin centrifuge na wannan kit ɗin sabon nau'in kayan abu ne na musamman ga kamfaninmu, wanda zai iya ɗaure DNA da kyau kuma musamman, kuma zai iya cire RNA, ƙazanta, sunadarai, ions da sauran kwayoyin halitta a cikin sel zuwa iyakar.Rukunin DNA da aka samu suna da girma, tsafta mai ƙarfi, kwanciyar hankali da ingantaccen inganci.Matsakaicin iyawar ginshiƙin DNA-kawai shine 80 μg DNA.Ana iya amfani da DNA ɗin da aka samu a cikin gwaje-gwajen ilimin halitta kamar narkewar enzyme, PCR, haɗaɗɗen kudanci da ginin ɗakin karatu.
Kit ɗin zai iya sarrafa har zuwa 1ml na jini a lokaci ɗaya don samun isassun adadin DNA mai tsabta mai tsabta.
Ƙayyadaddun bayanai
| Mini Kit na jini DNA | |||
| Abubuwan da ke ciki | Farashin 05111 | Farashin 05112 | Farashin 05113 |
| 50 T | 100 T | 250 T | |
| Farashin BL1 | ml 15 | ml 30 | ml 75 |
| Buffer BL2* | ml 15 | ml 30 | ml 75 |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| Buffer PW* | ml 25 | ml 50 | 125 ml |
| Farashin WB1 | ml 15 | ml 30 | ml 75 |
| Buffer EB | ml 10 | 20 ml | ml 50 |
| Foregene Protease Plus | 1.25 ml | 2.5 ml | 6.5 ml |
| Rukunin DNA-Kawai | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer BL2 da Buffer PW sun ƙunshi gishirin ruwa mai ban haushi.Da fatan za a sa safar hannu kuma ɗauki matakan kariya masu dacewa lokacin aiki.
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA na kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana guje wa gurɓatar da dakin gwaje-gwaje ta RNase na waje.
-Mai saurin sauri: Foregene Protease Plus yana da ayyuka mafi girma fiye da nau'ikan proteases iri ɗaya, kuma yana narkar da samfuran nama cikin sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 40.
-Daɗi: Ana yin centrifugation a dakin da zafin jiki, 4 ° C centrifugation ko ethanol hazo na DNA ba a buƙatar.
-Safety: Ba a yi amfani da hakar reagent na halitta ba.
-Maɗaukakiyar inganci: Gaɓar halittar DNA da aka fitar suna da girma, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da cirewa da tsarkakewa na DNA na genomic daga sabo ko daskararre anticoagulated jini duka.
Gudun aiki
Adana da rayuwar shiryayye
Ana iya adana wannan kit ɗin na tsawon watanni 12 a ƙarƙashin busassun yanayi a cikin zafin jiki (15-25 ° C);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
Maganin Foregene Protease Plus yana da tsari na musamman, wanda ke aiki lokacin da aka adana shi a dakin da zafin jiki na dogon lokaci (watanni 3);zai zama mafi aiki da kwanciyar hankali lokacin da aka adana shi a 4 ° C, don haka ana bada shawara don adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20 ° C.
