Tsarin Tsabtace PCR PCR Tsabtace Tsabtace Don DNA mai tsafta
Bayanin Kit:
Tsarkakewa da sauri da samun ɓangarorin DNA masu inganci daga tsarin PCR;Idan kuna son rabuwa da samun takamaiman gutsuttsuran DNA, da fatan za a zaɓi kayan dawo da gel.
◮ Faɗin farfadowar DNA: Ana iya dawo da gutsuttssun DNA masu gajeru kamar 60bp kuma masu girma kamar 10kb.
◮ Ingantaccen farfadowa: A dawo da inganci gabaɗaya fiye da 80%, kuma mafi girma zai iya kaiwa fiye da 95%.
◮ saurin gudu:Sauƙi don aiki, ana iya kammala dawo da guntun DNA a cikin mintuna 15 kuma ana iya cire dimer ɗin kai tsaye ba tare da dawo da gel ba.
◮ Sfety: Babu Organic reagent hakar da ake bukata.
◮ Kyakkyawan inganci: Gutsutsayen DNA da aka kwato suna da tsabta sosai, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
Bayani
Wannan kit ɗin yana amfani da ginshiƙin juzu'i da dabarar da kamfaninmu ya haɓaka, wanda zai iya dawo da tsaftataccen gutsuttsarin DNA daga tsarin PCR yadda yakamata.Rukunin tsarkakewa na iya ɗaure DNA da kyau, kuma an daidaita shi tare da reagent na musamman don cire dimer ɗin kai tsaye ba tare da dawo da gel ba.
Kit ɗin yana dawo da gutsuttsuran DNA da yawa.A ƙarƙashin yanayi na al'ada, ana iya dawo da gutsuttsuran DNA waɗanda gajeru kamar 60bp.Ana iya amfani da mafi ƙarancin 30μl na eluent don ƙara yawan tattara DNA da aka dawo dasu.
Kit ɗin yana da sauƙin aiki kuma yana da ƴan matakai.Yana buƙatar centrifugation da yawa kawai don aiwatar da samfurori da yawa a lokaci guda, kuma za'a iya samun tsattsauran tsaftar DNA da aka gano a cikin mintuna 15.
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Buffer BD* |
| Buffer BD-S |
| Farashin WB1 |
| Buffer EB |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Mai fa'ida na dawo da DNA: guntun DNA gajere kamar 60bp kuma girman 10kb ana iya dawo dasu.
-High farfadowa da aiki yadda ya dace: The dawo da yadda ya dace shi ne gaba ɗaya fiye da 80%, kuma mafi girma zai iya kai fiye da 95%.
-Mai saurin sauri: Sauƙi don aiki, ana iya kammala dawo da gutsuttsura DNA a cikin mintuna 15 kuma ana iya cire dimer ɗin kai tsaye ba tare da dawo da gel ba.
-Safety: Ba a buƙatar hakar reagent na halitta.
-Maɗaukaki mai inganci: Gutsun DNA da aka dawo dasu suna da tsafta, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
Kit aikace-aikace
Wannan kit ɗin ya dace don dawo da gutsuttsuran DNA (60bp-10kb) a cikin samfuran PCR.
Gudun aiki
Adana da Rayuwar Shelf
Ana iya adana wannan kit ɗin na tsawon watanni 12 a ƙarƙashin busassun yanayi a cikin zafin jiki (15-25 ° C);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
