Kasuwar DNA keɓewar Kits ɗin Tsabtacewa & Reagents don DNA na ƙasa
Bayanin Kit:
Tsarkakewa da sauri da samun DNA mai inganci mai inganci daga nau'ikan samfurin ƙasa daban-daban.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Bayani
Wannan kit ɗin yana ba da hanya mai sauri da sauƙi don fitar da DNA daga tushen ƙasa daban-daban.Akwai adadi mai yawa na masu hanawa kamar humic acid da ions ƙarfe a cikin samfuran ƙasa.Ko da waɗannan abubuwa sun kasance a cikin ƙananan kuɗi a cikin DNA mai tsabta, za su yi tasiri a kan halayen da ke ƙasa, kamar PCR da ƙuntataccen ƙwayar enzyme.Saboda haka, mabuɗin don tsarkake DNA na ƙasa shine yadda za a cire masu hanawa a cikin ƙasa yadda ya kamata.
Wannan kit ɗin yana amfani da DNA-Only Column wanda zai iya ɗaure musamman ga DNA, sabon-sabon Foregene Protease da kuma tsarin buffer na musamman, wanda zai iya cire masu hanawa daban-daban daga ƙasa yadda ya kamata ba tare da hakowar kwayoyin halitta ko ethanol da hazo na isopropanol ba.Ana iya kammala cire DNA daga samfuran ƙasa a cikin mintuna 90.
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 100, Shirye-shiryen 250
Abubuwan Kit
| Farashin SG1 |
| Farashin SG2 |
| Farashin SG3 |
| Farashin SG4 |
| Farashin PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Maganin Protease |
| Rukunin DNA-Kawai |
| Umarni |
Fasaloli & fa'idodi
-Babu gurɓatawar RNase: Rukunin DNA-kawai wanda kit ɗin ya samar yana ba da damar cire RNA daga DNA na kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana guje wa gurɓatar da dakin gwaje-gwaje ta RNase na waje.
Gudun sauri: Aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na ƙasa a cikin mintuna 90.
-Mafi dacewa: Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar 4°C low-zazzabi centrifugation ko ethanol hazo na DNA.
-Safety: Ba a buƙatar hakar reagent na halitta.
-Maɗaukakiyar inganci: DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
Kit aikace-aikace
Ya dace da tsarkakewar DNA na genomic daga samfurori masu zuwa: samfurori na silt, ƙasan gadon fure, ƙasa mai tattake ƙasa, ƙasan gandun daji, ƙasa mara kyau, ƙasa tafki, ƙasa lakeside, ƙasa lawn da sauran samfurori.
Gudun aiki
Adana da Rayuwar Shelf
-Za'a iya ajiye wannan kayan na tsawon watanni 12 a karkashin busasshen yanayi a cikin daki (15-25°C), idan ana bukatar a adana shi na tsawon lokaci, za'a iya adana shi a zazzabi na 2-8 ° C.
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Kafin amfani, tabbatar da sanya maganin a cikin kit a cikin zafin jiki na wani lokaci.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya shi kafin amfani.
-Foregene Protease bayani yana da tsari na musamman, wanda ke aiki lokacin da aka adana shi a dakin da zafin jiki na dogon lokaci (watanni 3);Ayyukansa da kwanciyar hankali za su fi kyau idan an adana su a 4 ° C, don haka ana bada shawara don adana shi a 4 ° C, ku tuna kada ku ajiye shi a -20 ° C.
-Dry Lysozyme foda yana adana a -20 ° C;An raba maganin Lysozyme da aka shirya zuwa ƙananan ƙananan kuma an adana shi a -20 ° C.
