Blood SuperDirectᵀᴹ PCR Kit (UNG) -Heparin Blood Direct PCR Master Mix don Genotyping na Jini
Bayanin Kit:
Kai tsaye yi amfani da heparin anticoagulated jinin gabaɗayan azaman samfuri don haɓaka PCR, tare da babban hankali da ingantaccen haɓakawa.An kara tsarin hana gurbatar yanayi na UNG don hana abubuwan da ba su dace ba.
◮Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini a hankali.
◮2× SuperEasyTMMix (UNG) - Haƙurin haƙuri na Heparin: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
◮Samfurin yana rufe cikakke, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.Tsarin PCR na rigakafi
◮2×SuperEasyTMMix (UNG), yadda ya kamata kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa, da tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Ƙayyadaddun bayanai
200 × 20μl rxns, 2000 × 20μl rxns
2× SuperEasyTMMix (UNG) -Heparin: A kan tushen 2 × SuperEasyTMMix-Heparin, ana amfani da dUTP maimakon dTTP, kuma ana ƙara UNG enzyme wanda zai iya lalata samfurin dUTP a lokaci guda, wanda zai iya hana gurbatar samfuran haɓaka PCR.Ya ƙunshi gyare-gyare na musamman na D-Taq DNA Polymerase, dNTPs, MgCl2, buffer dauki, PCR dauki, ingantawa da stabilizer, da dai sauransu.2O zuwa madaidaicin 2 × SuperEasyTM Mix (UNG) -Heparin da za a yi amfani da shi a cikin amsawar PCR.
6× DNA Loading Buffer: Buffer Loading bashi da SDS.Ana ba da shawarar yin amfani da 6x DNA Loading Buffer wanda aka haɗa tare da kit lokacin yin aikin agarose gel electrophoresis don samun sakamako mai kyau na electrophoresis.Kada a yi amfani da Loading Buffer mai ɗauke da SDS, in ba haka ba za a sami babban wutsiya mai haske a cikin layi yayin electrophoresis, wanda zai shafi sakamakon gwaji.
Abubuwan Kit
| 2× SuperEasyTMMix (UNG) - Heparin |
| 6× DNA Loading Buffer |
| Umarni |
Fasaloli & fa'idodi
-Tsarin yana da ƙarfin haɓakawa mai ƙarfi kuma yana iya gano kwayar halitta da ɓarnawar DNA na waje da ke cikin jini a hankali.
-2× SuperEasyTMMix (UNG) - Haƙurin haƙuri na Heparin: har zuwa 45% a cikin jinin ɗan adam da 20% a cikin jinin linzamin kwamfuta.
-Samfurin an rufe shi sosai, don haka babu buƙatar damuwa game da gurɓataccen samfurin da sakamakon PCR na ƙarya.Tsarin PCR na rigakafin gurɓatawa 2 × SuperEasyTMMix (UNG), yadda ya kamata kawar da gurɓataccen gurɓataccen abu da samfuran PCR ke haifarwa, da tabbatar da ƙayyadaddun ƙayyadaddun haɓakawa da daidaito.
Kit aikace-aikace
Ƙaddamarwa don kai tsaye ganewar PCR na Heparin anticoagulated jini duka.(Cikin hada da: jinin mutum, jinin bera, jinin kaza, jinin tsuntsu, jinin saniya, jinin kare, da sauransu).
Gudun aiki
Adana da Rayuwar Shelf
Dukkanin tsarin sufurin akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin yana cikin yanayin <4 ℃.
2× SuperEasyTMMix (UNG) - Heparin, idan ana amfani dashi akai-akai, ana iya adana shi a 4 ° C don ajiyar ɗan gajeren lokaci (amfani da shi cikin kwanaki 10).
6× DNA Loading Buffer, ana iya adana shi a 4°C ko -20°C na dogon lokaci.
