Kit ɗin keɓewar miRNA na dabba MicroRNA hakar da kayan tsarkakewa don Dabbobi
Bayanin Kit:
Da sauri kuma a fitar da ƙananan gutsuttsuran RNA na 20-200nt miRNA, siRNA, snRNA daga kyallen dabbobi da sel daban-daban.
◆Ana gudanar da dukkan tsarin a yanayin zafin daki (15-25 ° C), ba tare da wankan kankara da ƙananan zafin jiki ba.
◆Cikakken saiti na RNase-Free, babu buƙatar damuwa game da lalata RNA.
◆Rukunin Tsabtace DNA yana ɗaure musamman DNA, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara ƙarin DNA ba.
◆Babban yawan amfanin RNA: Rukunin RNA-kawai da dabara ta musamman na iya tsarkake RNA da inganci.
◆Gudun sauri: Aikin yana da sauƙi kuma ana iya kammala shi a cikin mintuna 30.
◆Tsaro: ba a buƙatar hakar reagents na halitta.
◆Babban inganci: Gutsunyoyin RNA da aka fitar suna da tsafta, ba su da furotin da sauran ƙazanta, kuma suna iya biyan buƙatun ƙasa.
◆Aikace-aikace na gwaji iri-iri
Bayani
Wannan kit ɗin yana amfani da ginshiƙin juzu'i da dabarar da kamfaninmu ya haɓaka, wanda zai iya fitar da ƙayyadaddun ɓangarorin RNA kamar 20-200nt miRNA, siRNA, snRNA daga kyallen dabbobi da sel daban-daban.Kit ɗin yana samar da DNA-Cleaning Column, wanda zai iya raba mai sauƙi daga lysate nama kuma ya cire DNA da RNA mai tsawo daga samfurin, wanda yake da sauƙi kuma yana adana lokaci;Rukunin RNA-kawai na iya ɗaure RNA da kyau tare da tsari na musamman, Zai iya aiwatar da adadi mai yawa na samfurori a lokaci guda.Dukkanin tsarin ba shi da RNase-Free, don kada miRNA mai tsafta ba ta lalacewa;Buffer miRW1, Tsarin wanke buffer miRW2, ta yadda RNA da aka samu ba ta da furotin, DNA, ions, da gurɓataccen mahalli.
Abubuwan Kit
| Keɓewar miRNA dabba Kit | ||||
| Abubuwan da ke ciki | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL* | 3ml ku | ml 25 | ml 50 | 100 ml |
| Buffer miRW1 | 4ml ku | ml 35 | ml 70 | ml 140 |
| Buffer miRW2 | 2.4ml ku | 24ml ku | ml 48 | ml 96 |
| RNase-Free ddH2O | 1.7ml ku | ml 10 | ml 20 | ml 40 |
| Rukunin RNA-kawai | 5 sets | 50sets | 100sets | 200sets |
| Rukunin Tsabtace DNA | 5 sets | 50sets | 100sets | 200sets |
| IFU | 1 yanki | 1 yanki | 1 yanki | 1 yanki |
Fasaloli & fa'idodi
◆Ana gudanar da dukkan tsarin a yanayin zafin daki (15-25 ° C), ba tare da wankan kankara da ƙananan zafin jiki ba.
◆Cikakken saiti na RNase-Free, babu buƙatar damuwa game da lalata RNA.
◆Rukunin Tsabtace DNA yana ɗaure musamman DNA, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara ƙarin DNA ba.
◆Babban yawan amfanin RNA: Rukunin RNA-kawai da dabara ta musamman na iya tsarkake RNA da inganci.
◆Gudun sauri: Aikin yana da sauƙi kuma ana iya kammala shi a cikin mintuna 30.
◆Tsaro: ba a buƙatar hakar reagents na halitta.
◆Babban inganci: Gutsunyoyin RNA da aka fitar suna da tsafta, ba su da furotin da sauran ƙazanta, kuma suna iya biyan buƙatun ƙasa.
◆Aikace-aikace na gwaji iri-iri
Kit aikace-aikace
Ya dace da hakar da tsarkakewar miRNA daga sabo ko daskararru iri-iri na kyallen jikin dabba ko sel.
Adana da Rayuwar Shelf
Ana iya adana wannan kit ɗin na tsawon watanni 24 a ƙarƙashin busassun yanayi a zafin jiki (15-25 ° C), idan ana buƙatar adana shi na dogon lokaci, ana iya adana shi a2-8 ° C.Ana iya sanya buffer miRL a4°Cna wata 1 bayan karawaβ-mercaptoethanol(an bada shawarar ƙara shi yanzu).
