Lnc-RT Heroᵀᴹ I (Tare da gDNase) (Super Premix don haɗin cDNA na farko daga lncRNA)
Ƙayyadaddun bayanai
25×20μl Rxn, 50×20μl Rxn
Lnc-RT HeroTM I (Tare da gDNase) tsarin juzu'i ne wanda aka haɓaka musamman don lncRNA don cire gurɓataccen DNA da sauri.5×gDNase Mix zai iya hanzarta cire ragowar kwayoyin halitta a cikin RNA a 42°C na mintuna 2, yadda ya kamata ya guje wa tsoma bakin kwayoyin halitta akan sakamakon qPCR.
5 × L-RT HeroTM Mix yana ƙunshe da Foregene LncRNA Reverse Transcriptase musamman wanda Foregene ya haɓaka, wanda shine sabon nau'in juzu'i na juzu'i na musamman don lncRNA da sauran samfuran hadaddun RNA masu tsayi, tare da ƙaƙƙarfan alaƙar RNA da ingantaccen rikodin juyewa.Ingantaccen tsarin yana sa jujjuya juzu'i cikin sauri kuma yana iya sauƙin kwafin samfuran RNA tare da babban abun ciki na GC da hadadden tsarin sakandare.Za'a iya kammala haɗin cDNA na farko a 42°C a cikin mintuna 15.
Abubuwan Kit
| 5× gDNase Mix |
| 5× L-RT HeroTM Mix |
| RNase-Free ddH2O |
| Umarni |
Fasaloli & fa'idodi
■Ingantacciyar ikon cire gDNA, wanda zai iya cire gDNA a cikin samfuri a cikin mintuna 2.
Zai iya juyar da rubutun lncRNA yadda ya kamata.Lokacin da samfurin juzu'i ya kasance ƙarƙashin qPCR, ƙimar Ct ta yi ƙasa da na tsarin rubutun juyi na al'ada.
∎ Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 15 ne kawai don kammala haɗin layin farko na cDNA.
∎ Haɗaɗɗen samfura: samfuran da ke da babban abun ciki na GC da hadadden tsari na biyu kuma ana iya juyar da su tare da babban inganci.
∎ Tsarin juzu'i mai girman hankali, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
∎ Tsarin rubutun juyi yana da babban kwanciyar hankali na zafi, mafi kyawun yanayin zafi shine 42℃, kuma har yanzu yana da kyakkyawan aikin juzu'i a 50℃.
Kit aikace-aikace
lncRNA dangi ƙididdigar ƙididdiga.
lncRNA cikakken ƙididdigar ƙididdigewa.
Yana iya sauri da kuma daidai tantance alamar RNA kamar kwayar cutar RNA.
Juya rubutun samfuran RNA tare da babban abun ciki na GC ko hadadden tsarin sakandare.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana kayan a -20°C. Ajiye samfurin a cikin firiji mai yawan zafin jiki a -20°C nan da nan bayan an karɓa.Idan yanayin ajiya ya dace, samfur ɗin ba zai ƙasƙantar da kowane aiki ba yayin lokacin ingancin shekara 1.
Jagorar Binciken Matsala
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-ƙayyadaddun haɓakawa
1. Zane na farko mara ma'ana
Shawarwari: Ƙirar ƙirar ƙira bisa ga ƙa'idodin ƙirar ƙira.
2. Ragowar kwayoyin halitta.
Shawarwari: Tabbatar cewa matakin farko na zazzabi na cire gDNA shine 42°C, kuma ana iya tsawaita lokacin amsawa zuwa 5min.
Babu siginar ƙarawa ta RT-qPCR
1. RNA ya lalace.
Shawarwari: Ya kamata kayan aikin hakar RNA ya zama sabo sosai gwargwadon yiwuwa, kuma ya kamata a yi amfani da RNA mai inganci da tsafta.
2. RNA ya ƙunshi masu hanawa.
Shawarwari: Masu hana fassarar juzu'i gabaɗaya sun haɗa da SDS, gishirin guanidine, EDTA, da sauransu. Ana ba da shawarar a wanke RNA tare da 70% ethanol don cire masu hanawa.
3. Abubuwan ƙira na farko.
Shawarwari: Dangane da ƙa'idar ƙira ta farko, sake sake fasalin abubuwan da aka tsara don dubawa.
Ƙungiyar da aka yi niyya tana bayyana a cikin madaidaicin iko
1. Gurɓatar kayan aikin aiki ko reagents.
Shawarwari: Duk reagents ko kayan aikin gwajin yakamata a ƙera su ta atomatik.Yi hankali da tausasawa yayin mu'amala don hana samfuran DNA tsotsa cikin pipette ko zubewa daga bututun centrifuge.
2. Gurɓatawa ya faru a lokacin shirye-shiryen tsarin amsawar PCR.
Shawarwari: Ɗauki matakan da suka wajaba lokacin da ake mu'amala, kamar: saka safofin hannu na latex, ta amfani da tukwici na tacewa.Yi amfani da Haɗin PCR na Real Time a cikin tsarin ƙaƙƙarfan ƙazanta.
3. Abubuwan farko sun ƙasƙanta.
Shawara: Yi amfani da SDS-PAGE electrophoresis don gano ko abubuwan da aka lalata sun lalace, kuma a musanya su da sabbin maƙallan don gwaje-gwajen gano haske.
Jagoran Jagora:
Lnc-RT HeroTM I (Tare da gDNase) (Super Premix don haɗin cDNA na farko daga lncRNA) Manual umarni
