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Foreasy HS Taq DNA Polymerase

Haɓaka Hoton HS Taq DNA Polymerase
  • Foreasy HS Taq DNA Polymerase

Bayanin Kit:

◮Babban ƙayyadaddun ƙayyadaddun abu: Enzyme tare da babban aikin farawa mai zafi.

◮Saurin haɓakawa: 10 sec/kb.

◮Babban samfuri na daidaitawa: ana iya amfani da shi don haɓaka High sosaiGCdarajakumasamfuri na DNA daban-daban masu wahala-da-girma.

◮Aminci mai ƙarfi: Amincin shine sau 6of talakawa Taq Enzyme.

karfin gaba


Cikakken Bayani

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FAQ

Bayani

Foreasy HS Taq DNA Polymerase shine sabon Taq enzyme wanda aka bayyana a cikin kwayoyin Escherichia coli injiniya ta hanyar fasahar sake hadewar kwayoyin halitta.Bayan an bi da enzyme tare da tsari na musamman, shi'Ana hana ayyukan s kafin kunna zafin zafi, don haka hana haɓakar da ba takamammen takamammen abin da ya haifar da rashin ƙayyadaddun ƙayyadaddun buƙatu na alƙawari ko dimers a ƙarƙashin ƙarancin yanayin zafi.Wannan samfurin ya dace da takamaiman amsawar PCRion, M ultiple x PCR , babban abun ciki na GC (> 60%),tare datsarin sakandareko kuma wasukarfi baya genomicshaɓakawa da babban sikelin genomicsgano haɓakawa.Enzyme yana da 5' 3' Ayyukan DNA polymerase da 5' → 3' ayyukan exonuclease, amma babu 3' → 5' aikin exonuclease.

Abubuwan Kit

Bangaren Saukewa: IM-01021 Saukewa: IM-01022 Saukewa: IM-01023
HS Taq DNA Polymerase (5 U/μL)  5000 U (1 ml)  50 KU (10 ml)  500 KU (100 ml)
2× Taq Reaction Buffer  25ml × 5  250 ml × 5  500 ml × 25

Fasaloli & fa'idodi

- Babban ƙayyadaddun ƙayyadaddun abu: Enzyme tare da babban aikin farawa mai zafi.

- Saurin haɓakawa: 10 sec/kb.

- Babban daidaitawa na samfuri: ana iya amfani da shi don haɓaka High sosaiGCdarajakumasamfuri na DNA daban-daban masu wahala-da-girma.

- Karfin aminci: Amincinta shine sau 6of talakawa Taq Enzyme.

Kit aikace-aikace

- Tsarin PCR / qPCR daban-daban da tsarin PCR kai tsaye

- PCR Ƙarfafa DNA Fragment

- alamar DNA

- Tsarin DNA

- PCR da A wutsiya

Ma'anar Ayyuka

1U: Adadin enzyme da ake buƙata don haɗa 10 nmol naDNAcikin al'amarin acid-insoluble ta hanyar amfani da DNA na maniyyi mai kunnawa azaman samfuri / firamare, 74 ° C, mintuna 30.

Halin Amsa

Zazzabi Lokacin Amsa Lokacin zagayowar
37°C 5 min 1
94°C 5 min 1
94°C Dakika 10  40
60°C Dakika 10

Lura:Don tsarin 10 µL da 20 µL, ƙara daidai adadin mai na ma'adinai idan mai hawan zafi ba shi da murfi mai zafi.

Yanayin amsawa na PCR ya bambanta dangane da yanayin tsarin samfuri, firamare, da makamantansu.A cikin takamaiman aiki, ya zama dole don ƙirƙira mafi kyawun yanayin amsawa, gami da zafin jiki na annealing, lokacin tsawaitawa, da sauransu, bisa ga takamaiman yanayi kamar nau'in samfuri, girman guntun manufa, jerin tushe na guntu mai ƙarfi, da abun ciki na GC da tsayin firam.

Adana

-20 ± 5 °C na shekaru 2 ko a -80 °C don adana dogon lokaci.

  • Na baya:
  • Na gaba:

    • Babu alamun ƙarawa

    1.Taq DNA Polymerase a cikin kit ɗin ya rasa aikinsa saboda rashin ajiya mai kyau ko ƙarewar kit ɗin.
    Shawarwari: Tabbatar da yanayin ajiya na kit;sake ƙara adadin da ya dace na Taq DNA Polymerase zuwa tsarin PCR ko siyan sabon Kit ɗin PCR na Real Time don gwaje-gwaje masu alaƙa.

    2.Akwai masu hanawa da yawa na Taq DNA Polymerase a cikin samfurin DNA.
    Shawara: Gyara samfuri ko rage adadin samfurin da aka yi amfani da shi.

    3.The Mg2 + taro bai dace ba.
    Shawarwari: Matsakaicin Mg2+ na 2 × Real PCR Mix da muke samarwa shine 3.5mM.Koyaya, ga wasu firamare na musamman da samfura, ƙaddamarwar Mg2+ na iya zama mafi girma.Don haka, zaku iya ƙara MgCl2 kai tsaye don haɓaka taro na Mg2+.Ana ba da shawarar ƙara Mg2+ 0.5mM kowane lokaci don ingantawa.

    4.The PCR amplification yanayi ba su dace, da kuma firamare jerin ko maida hankali ne ba daidai ba.
    Shawarwari: tabbatar da daidaiton jerin abubuwan farko kuma ba a ƙasƙantar da na'urar ba;idan siginar ƙarawa ba ta da kyau, gwada rage yawan zafin jiki da daidaita ma'auni daidai.

    5.Yawan samfuri ya yi kadan ko da yawa.
    Shawarwarin: Yi samfuri na daidaitawa gradient dilution, kuma zaɓi ƙaddamarwar samfuri tare da mafi kyawun tasirin PCR don gwajin PCR na Real Time.

    NTC yana da ƙimar haske mai girma sosai

    1.Reagent gurbatawa lalacewa ta hanyar aiki.
    Shawarwari: Sauya tare da sababbin reagents don gwajin PCR na Real Time.

    2.Contamination ya faru a lokacin shirye-shiryen tsarin amsawar PCR.
    Shawarwari: Ɗauki matakan kariya masu mahimmanci yayin aiki, kamar: sanya safofin hannu na latex, yin amfani da tip ɗin pipette tare da tacewa, da sauransu.

    3.The primers suna ƙasƙantar da kai, kuma lalatawar abubuwan da aka yi amfani da su za su haifar da haɓakar da ba ta dace ba.
    Shawara: Yi amfani da SDS-PAGE electrophoresis don gano ko abubuwan da aka lalata sun lalace, kuma a maye gurbin su da sabbin maƙallan don gwajin PCR na Real Time.

    Dimer na farko ko haɓakawa mara takamaiman

    1.The Mg2 + taro bai dace ba.
    Shawarwari: Tsarin Mg2+ na 2 × Real PCR EasyTM Mix da muke samarwa shine 3.5 mM.Koyaya, ga wasu firamare na musamman da samfura, ƙaddamarwar Mg2+ na iya zama mafi girma.Don haka, zaku iya ƙara MgCl2 kai tsaye don haɓaka taro na Mg2+.Ana ba da shawarar ƙara Mg2+ 0.5mM kowane lokaci don ingantawa.

    2.The PCR annealing zafin jiki ne ma low.
    Shawara: Ƙara yawan zafin jiki na PCR da 1℃ ko 2℃ kowane lokaci.

    3.A samfurin PCR yayi tsayi da yawa.
    Shawarwari: Tsawon samfurin PCR na Real Time yakamata ya kasance tsakanin 100-150bp, bai wuce 500bp ba.

    4.The primers suna ƙasƙanci, kuma lalatawar abubuwan da aka tsara za su haifar da bayyanar ƙayyadaddun haɓakawa.
    Shawara: Yi amfani da SDS-PAGE electrophoresis don gano ko abubuwan da aka lalata sun lalace, kuma a maye gurbin su da sabbin maƙallan don gwajin PCR na Real Time.

    5.Tsarin PCR bai dace ba, ko tsarin ya yi ƙanƙanta.
    Shawara: Tsarin amsawar PCR ya yi ƙanƙanta zai sa daidaiton ganowa ya ragu.Zai fi kyau a yi amfani da tsarin amsawa da kayan aikin PCR masu ƙima suka ba da shawarar don sake gudanar da gwajin PCR na Real Time.

    Rashin maimaita ƙimar ƙididdiga

    1. Kayan aiki yana da matsala.
    Shawara: Ana iya samun kurakurai tsakanin kowane rami na PCR na kayan aiki, wanda ke haifar da rashin haɓakawa yayin sarrafa zafin jiki ko ganowa.Da fatan za a bincika bisa ga umarnin kayan aikin da ya dace.

    2.Tsarin samfurin ba shi da kyau.
    Shawarwari: Samfuran da ba su da tsabta za su haifar da rashin daidaituwa na gwaji, wanda ya haɗa da tsarkin samfuri da masu farawa.Zai fi dacewa don sake tsarkake samfuri, kuma masu farawa sun fi tsarkakewa ta SDS-PAGE.

    3.The PCR tsarin shirye-shiryen da lokacin ajiya ya yi tsayi da yawa.
    Shawara: Yi amfani da tsarin PCR na ainihi don gwajin PCR nan da nan bayan shiri, kuma kar a bar shi a gefe na dogon lokaci.

    4.The PCR amplification yanayi ba su dace, da kuma firamare jerin ko maida hankali ne ba daidai ba.
    Shawarwari: tabbatar da daidaiton jerin abubuwan farko kuma ba a ƙasƙantar da na'urar ba;idan siginar ƙarawa ba ta da kyau, gwada rage yawan zafin jiki da daidaita ma'auni daidai.

    5.Tsarin PCR bai dace ba, ko tsarin ya yi ƙanƙanta.
    Shawara: Tsarin amsawar PCR ya yi ƙanƙanta zai sa daidaiton ganowa ya ragu.Zai fi kyau a yi amfani da tsarin amsawa da kayan aikin PCR masu ƙima suka ba da shawarar don sake gudanar da gwajin PCR na Real Time.

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