RT Easyᵀᴹ I Master Premix don haɗakarwar cDNA na farko
Bayanin Kit:
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 25 ne kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃,kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 45%, gishiri guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
Bayanin Kit
FOREGENE's 2 × RT SauƙiTMCakuda na iya haɗa cDNA-na farko a zazzabi mai girma kamar 50°C, wanda ke da tasiri ga juyar da amsawar juzu'i na rikitaccen tsarin RNA na biyu.
RNA da aka tsarkake a cikin dakin gwaje-gwaje sau da yawa ya ƙunshi barasa da ragowar gishiri na guanidine, waɗanda ke da kaddarorin hanawa masu ƙarfi akan mafi yawan juzu'i na baya, yana haifar da rashin gamsuwa da tasirin juzu'i ko ƙarancin juzu'i mai inganci.FOREGENE 2× RT SauƙiTMMix yana nuna babban juriya ga barasa da gishirin guanidine, mafi girman juriya ga barasa a samfuran RNA shine 45%, kuma mafi girman haƙuri ga gishirin guanidine shine 750mM.Ko da RNA ba ta da tsarki, ana iya yin juzu'in juzu'i tare da FORREGENE 2×RT Mix.Tsarin amsawa na musamman yana sa amsawar RT ta kasance mai sauƙi, sauri da inganci, kuma ana iya kammala haɗakar cDNA na farko a cikin mintuna 25.
Ana iya amfani da wannan samfurin tare da haɗin gwiwar Foregene's fluorescent quantitative samfurin Real Time PCR EasyTMdon samun sakamako na gwaji mai inganci.
Ƙayyadaddun bayanai
| RT SauƙiTMI Jagoran Premix don haɗawar cDNA-na farko | ||
| Abubuwan da ke ciki | RT-01011 | RT-01012 |
| 25 sau (20μl tsarin) | 100 sau (20μl tsarin) | |
| 2× RT EasyTM Mix | 0.25 ml | 1 ml |
| Random Primer (50μM) | 50ml ku | 200 μl |
| Oligo (dT) 18 Firamare (50μM) | 50ml ku | 200 μl |
| RNase-Free ddH2O | 1.7ml ku | 1.7ml ku |
| Jagoran Jagora | guda 1 | guda 1 |
Fasaloli & fa'idodi
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 25 ne kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da babban kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃, kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 45%, gishiri guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
Kit aikace-aikace
- RT-PCR na al'ada
- Haɗin cDNA don bincike na cloning da magana
- Bincike na hanyar tsawaita matakin farko na wurin ƙaddamar da rubutun
- RACE (ƙarfafa haɓakar cDNA da sauri)
- Ƙarfafa RNA na layi
- Chip mark
Gudun aiki
Adana da rayuwar shiryayye
1.Yanayin sufuri
Dukkanin tsarin sufurin akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin yana cikin yanayin <4 ℃.
2. Yanayin ajiya
RT SauƙiTMAna adana ni a -20 ° C.Ajiye samfurin a cikin firiji mai yawan zafin jiki a -20°C nan da nan bayan an karɓa.Idan yanayin ajiya ya dace, samfur ɗin ba zai ƙasƙantar da kowane aiki ba yayin lokacin ingancin shekara 1.
