RT Easyᵀᴹ II Master Premix don haɗawar cDNA na farko don PCR na ainihi
Bayanin Kit:
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 20 kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da babban kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃, kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 60%, gishirin guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
- 2× RT KO-SaukiTM Mix ya ƙara ingantattun abubuwan rubutu na baya (Random Primer, Oligo(dT)18 Farko).
Bayanin Kit
Foregene's 2 × RT OR-SaukiTMCakuda na iya haɗa cDNA-na farko a zazzabi mai girma kamar 50°C, wanda ke da tasiri ga juyar da amsawar juzu'i na rikitaccen tsarin RNA na biyu.
RNA da aka tsarkake a cikin dakin gwaje-gwaje sau da yawa ya ƙunshi barasa da ragowar gishiri na guanidine, waɗanda ke da kaddarorin hanawa masu ƙarfi akan mafi yawan juzu'i na baya, yana haifar da rashin gamsuwa da tasirin juzu'i ko ƙarancin juzu'i mai inganci.Foregene 2× RT Mix yana nuna matuƙar haƙuri ga barasa da gishirin guanidine.Mafi girman juriya ga barasa a samfuran RNA shine 60%, kuma mafi girman haƙuri ga gishirin guanidine shine 750mM.Ko da RNA mara tsarki za a iya amfani da shi don mayar da martanin rubutu tare da Foregene 2 × RT OR-SaukiTMMix
2× RT KO-SaukiTMMix shine saurin juzu'i mai jujjuyawa reagent wanda aka haɓaka musamman don PCR na Real Time.Wannan tsarin yana da ingantaccen juzu'i na juzu'i kuma baya buƙatar ƙara kowane firamare, kuma yana iya aiwatar da halayen juzu'i masu kyau akan ƙaramin adadin samfuran RNA.Tsarin amsawa na musamman yana sa amsawar RT ta fi sauƙi, sauri da inganci.Za'a iya kammala haɗin cDNA na farko a cikin mintuna 20.Ana iya amfani da wannan samfurin tare da haɗin gwiwar Foregene's fluorescent quantitative samfurin Real Time PCR EasyTMdon samun sakamako na gwaji mai inganci.
Ƙayyadaddun bayanai
| RT SauƙiTMII Jagora Premix don haɗawar cDNA na farko don PCR na Real Time | |||
| Abubuwan Kit | RT-01021 | RT-01022 | RT-01023 |
| 50T (10 μl tsarin) | 200 T (10 μl tsarin) | 800T (tsarin 10 μl) | |
| 2× RT KO-SaukiTMMix | 0.25 ml | 1 ml | 1ml × 4 |
| RNase-Free ddH2O | 1.7ml ku | 1.7ml ku | 1.7ml × 3 |
| Jagoran Jagora | guda 1 | guda 1 | guda 1 |
Fasaloli & fa'idodi
- Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 20 kawai don kammala haɗin layin farko na cDNA.
- Babban tsarin jujjuya juzu'i, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
- Tsarin rubutun juyi yana da babban kwanciyar hankali na thermal, yanayin zafin tsarin na iya zama sama da 50 ℃, kuma yana da kyakkyawan aikin juzu'i.
- Hatta samfuran RNA na ƙazanta (giya har zuwa 60%, gishirin guanidine har zuwa 750mM) ana iya juyar da martanin rubutu.
- 2× RT KO-SaukiTMMix ya ƙara ingantattun abubuwan rubutu na baya (Random Primer, Oligo(dT)18Farko).
Kit aikace-aikace
- An yi amfani da shi kai tsaye a cikin PCR na Real Time don tantance maganganun kwayoyin halitta
- Yana iya sauri da daidai tantance ƙananan adadin RNA kamar ƙwayoyin cuta na RNA
- Juya rubutun samfuran RNA tare da babban abun ciki na GC ko hadadden tsarin sakandare
Gudun aiki
Adana da rayuwar shiryayye
1. Yanayin sufuri
Dukkanin tsarin jigilar akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin yana cikin yanayin <4°C.
2. Yanayin ajiya
RT SauƙiTMII ana adana a -20 ° C.Ajiye samfurin a cikin firiji mai yawan zafin jiki a -20°C nan da nan bayan an karɓa.Idan yanayin ajiya ya dace, samfur ɗin ba zai ƙasƙantar da kowane aiki ba yayin lokacin ingancin shekara 1.
