Sarrafa-CoV-2 Kayan Gane Acid Nucleic (Tsarin Binciken Fluorescent PCR Multiplex)
Bayanin Kit:
PCR kai tsaye:Babu buƙatar kayan cirewar acid nucleic da Tsarin, an kammala samfurin 96 a cikin awa 1 ko makamancin haka.
◮ Ƙananan buƙatun kayan aiki da sauƙin amfani:Kawai buƙatar tsarin PCR na Real-Time .
◮ LoD da babban hankali:Hankalin ganowa zai iya kaiwa mafi ƙanƙanta matakin kamar kwafi 500/ml.
Amfani da Niyya
Wannan kit ɗin yana amfani da fasahar RT PCR ta Real-time (rRT-PCR) don gano ƙimar nucleic acid na SARS-CoV-2 a cikin samfuran nasopharyngeal na ɗan adam ko samfuran swab na oropharyngeal.
Ka'idar gwaji
An ƙirƙira wannan ƙirar farko da saitin bincike don gano jerin abubuwan da aka adana na SARS-CoV-2 (ORF1ab gene da N gene).Kit ɗin ya ƙunshi Ikon Ciki wanda ake amfani dashi don tantance ingancin samfur.
Fasahar PCR ta Real-Time tana amfani da tsarin sarkar polymerase (PCR) don haɓaka takamaiman manufa
jeri da niyya takamaiman bincike don gano RNA mai ƙarfi.Ana yiwa masu binciken lakabi da su
mai ba da labari mai kyalli da rini mai kashewa.
Cikakken Bayani
◮Ƙayyadaddun bayanai:48 Rxns/kit, 96 Rxns/kit
◮ Takamaiman:96.72%
Hankali:97.92%
◮ Nau'in samfuri masu amfani
Nasopharyngeal Swab ko Oropharyngeal Swab
◮Kayan aiki mai dacewa
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Abubuwan da aka gyara
| A'a. | Bangaren | Adadin | Manyan abubuwan da aka gyara | |||
| 48 rxn | 96 rxn | |||||
| 1 | Nucleic acid wakili saki | 1.4ml / tube | 2 bututu | 5.3 ml / kwalba | kwalba 1 | Surfactant |
| 2 | Mai kare RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Mai hana RNase |
| 3 | Maganin amsawar SARS-CoV-2 | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Firamare, bincike, mayar da martani, dNTP |
| 4 | SARS-CoV-2 enzyme cakuda | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Hot fara Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 tabbatacce iko | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid dauke da guntun manufa, RNA |
| 6 | SARS-CoV-2 korau iko | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Fasaloli & fa'idodi
◮Farashin PCR
Babu buƙatar kayan cirewar acid nucleic da Tsarin, an kammala samfurin 96 a cikin awa 1 ko makamancin haka.
◮Ƙananan buƙatun kayan aiki da sauƙin amfani sosai
Kawai buƙatar tsarin PCR na Real-Time.
◮LoD da babban hankali
Hankalin ganowa zai iya kaiwa mafi ƙanƙanta matakin kamar kwafi 500/ml.
Yadda ake amfani da shi
Hanyar 1: Hanyar PCR kai tsaye
Hanyar 2: Kwayar cuta ta RNA Keɓewa + PCR
Hanyar 1: Hanyar PCR kai tsaye ta amfani daSamfurin Sakin Wakilin
Hanyar ganowa
Jirgin Jirgin Ruwa da Ajiye
An rufe shi daga haske kuma an adana shi a -20 ± 5 ℃;
Rayuwar rayuwa
shekara 1
Bayanin oda
Bidiyon Ayyukan gaggawa
Ku rubuta sakonku anan ku aiko mana
