Kayan Keɓancewar DNA na Kwayar cuta da RNA Viral DNA da RNA Extraction Preparation Preparation Kits
Ƙayyadaddun bayanai
Shirye-shirye 50, Shirye-shiryen 200
Viral RNA Nucleic acid Extraction Extraction Kit yana amfani da ginshiƙin juzu'i da dabara wanda Foregene ya haɓaka, wanda zai iya fitar da ingantaccen RNA mai inganci da inganci daga samfura kamar plasma, jini, ruwan jiki mara-kyau, da al'adun tantanin halitta.Kit ɗin yana ƙara musamman Linear Acrylamide, wanda zai iya ɗaukar ƙananan adadin RNA cikin sauƙi daga samfuran.Rukunin RNA-Kawai na iya ɗaure RNA da nagarta sosai.Kit ɗin na iya aiwatar da babban adadin samfurori a lokaci guda.
Gabaɗayan kit ɗin bai ƙunshi RNase ba, don haka tsarkakewar RNA ba zai ƙasƙanta ba.Buffer viRW1 da Buffer viRW2 na iya tabbatar da cewa acid nucleic da aka samu ba shi da furotin, nuclease ko wasu ƙazanta, waɗanda za a iya amfani da su kai tsaye don gwaje-gwajen nazarin halittu na ƙasa.
Abubuwan Kit
| Acrylamide Linear |
| Farashin DRL |
| Buffer RW1, Mai Buffer RW2 |
| RNase-Free ddH2O |
| Rukunin DNA/RNA |
| Umarni |
Fasaloli & fa'idodi
■ Aiki a dakin da zafin jiki (15-25 ℃) a ko'ina cikin tsari, ba tare da wankan kankara da ƙananan zafin jiki ba.
n Cikakken kayan aikin RNase-Free, babu buƙatar damuwa game da lalata RNA.
∎ Yawan yawan adadin acid nucleic: Rukunin DNA/RNA-kawai da tsari na musamman na iya tsarkake DNA da RNA yadda ya kamata.
∎ Babban ƙarfin sarrafa samfurin: ana iya sarrafa samfuran har zuwa 200μl kowane lokaci.
n Gudun sauri: mai sauƙin aiki kuma ana iya kammala shi cikin mintuna 20.
∎ Tsaro: ba a bužatar sinadarin reagents.
∎ Babban inganci: gutsuttssun RNA da aka tsarkake suna da tsafta, ba su da furotin da sauran ƙazanta, kuma suna iya saduwa da aikace-aikacen gwaji daban-daban.
Kit aikace-aikace
Ya dace da hakar da tsarkakewa na kwayar nucleic acid a cikin samfurori irin su plasma, serum, ruwan jiki marar tantanin halitta da al'adar tantanin halitta.
Gudun aiki
zane
Adana da Rayuwar Shelf
■ Ana iya adana wannan kit ɗin na tsawon watanni 24 a ƙarƙashin busassun yanayi a zafin jiki (15-25 ℃);Idan ana buƙatar adana na dogon lokaci, ana iya adana shi a cikin 2-8 ℃.
■ Za a iya adana maganin Acrylamide na Linear a zafin jiki na kwanaki 7;Bayan karbar kayan, da fatan za a fitar da shi kuma adana shi a zazzabi na -20 ° C.
Bayan ƙara Linear Acrylamide zuwa Buffer DRL, ana iya adana shi a 2-8°C har zuwa awanni 48.Da fatan za a yi amfani da ingantaccen bayani.
Jagorar Binciken Matsala
Mai zuwa shine nazarin matsalolin da za'a iya fuskanta a cikin hakar DNA/RNA na hoto, da fatan ya taimaka ga gwaje-gwajenku.Bugu da ƙari, don wasu matsalolin gwaji ko fasaha ban da umarnin aiki da bincike na matsala, mun ƙaddamar da tallafin fasaha don taimaka muku.Idan kuna da wasu buƙatu, tuntuɓe mu: 028-83360257 ko imel:
Tech@foregene.com.
Babu haɓakar acid nucleic ko ƙarancin ƙwayar nucleic acid
Yawancin lokaci akwai dalilai da yawa waɗanda ke shafar haɓakar farfadowa, kamar: samfurin abun ciki na nucleic acid, hanyar aiki, ƙarar elution, da sauransu.
Binciken abubuwan gama gari:
1. An yi wanka na kankara ko ƙananan zafin jiki (4 ° C) centrifugation yayin aikin.
Shawarwari: Yi aiki a dakin da zafin jiki (15-25°C) a duk tsawon tsari, kar a yi wanka da ƙarancin zafin jiki.
2. An adana samfurin ba daidai ba ko kuma an adana samfurin na dogon lokaci.
Shawarwari: Ajiye samfurori a -80 ° C kuma kauce wa daskarewa da narke maimaitawa;yi ƙoƙarin yin amfani da sabbin samfuran da aka tattara don hakar acid nucleic.
3. Rashin isasshen samfurin lysis.
Shawarwari: Da fatan za a tabbatar da cewa samfurin da maganin aikin lysis an gauraye su sosai kuma an sanya su a cikin zafin jiki (15-25 ° C) na minti 10.
4. Ƙarin kuskure na eluent.
Shawara: Tabbatar cewa an ƙara RNase-Free ddH2O a nitse zuwa tsakiyar membrane ginshiƙin tsarkakewa, kuma kar a jefa shi akan zoben ginshiƙin tsarkakewa.
5. Ba a ƙara madaidaicin ƙarar cikakken ethanol zuwa Buffer RW2 ba.
Shawara: Da fatan za a bi umarnin, ƙara madaidaicin ƙarar cikakken ethanol zuwa Buffer RW2 kuma a haɗe da kyau kafin amfani da kit ɗin.
6. Ƙarfin samfurin da bai dace ba.
Shawarwari: Ana sarrafa 200µl na samfurin don kowane 500µl na Buffer DRL.Matsakaicin sarrafa samfurin zai haifar da ƙananan haɓakar haɓakar acid nucleic.
7. Ƙarfin ƙira mara dacewa ko rashin cikawa.
Shawarwarin: Ƙarfin eluent na ginshiƙin tsarkakewa shine 30-50μl;idan tasirin elution bai gamsar ba, ana ba da shawarar tsawaita lokacin a cikin zafin jiki bayan ƙara ddH2O da aka rigaya da RNase-Free, kamar 5-10min.
8. Ethanol ya kasance a kan ginshiƙi bayan wankewa tare da Buffer RW2.
Shawara: Idan ethanol ya kasance bayan centrifugation tare da Buffer RW2 na mintuna 2, ana iya sanya ginshiƙi a cikin zafin jiki na minti 5 bayan centrifugation don cire ƙarancin ethanol gabaɗaya.
Acid nucleic da aka tsarkake ya lalace
Ingancin tsararren nucleic acid yana da alaƙa da adana samfurin, gurɓataccen RNase, aiki da sauran dalilai.Binciken abubuwan gama gari:
1. Ba a adana samfuran da aka tattara a cikin lokaci ba.
Shawara: Idan ba a yi amfani da samfurin a cikin lokaci bayan tattarawa ba, da fatan za a adana shi a -80 ° C a ƙananan zafin jiki nan da nan.Don hakar RNA, gwada amfani da samfuran da aka tattara sabo.
2. Tattara samfuran kuma daskare kuma a narke akai-akai.
Shawara: Guji daskarewa da narke (ba fiye da sau ɗaya ba) yayin tattarawa da adana samfuran, in ba haka ba za a rage yawan amfanin acid nucleic.
3. An gabatar da RNase a cikin dakin aiki ko safofin hannu, abin rufe fuska, da sauransu.
Shawarwari: Gwajin cirewar RNA an fi yin su a cikin wani dakin aiki na RNA daban, kuma yakamata a tsaftace teburin dakin gwaje-gwaje kafin gwaji.
Sanya safar hannu da abin rufe fuska yayin gwaji don guje wa lalatawar RNA wanda ya haifar da gabatarwar RNase zuwa mafi girma.
4. An gurbata reagent da RNase yayin amfani.
Shawara: Sauya da sabon Viral DNA/RNA Keɓe Kit don gwaje-gwaje masu alaƙa.
5. Bututun centrifuge da tukwici na pipette da aka yi amfani da su don magudin RNA sun gurbata da RNase.
Shawara: Tabbatar cewa bututun centrifuge, tukwici na pipette, pipettes, da sauransu. da ake amfani da su don hakar RNA duk ba su da RNase.
Tsarkake acid nucleic yana shafar gwaje-gwaje na ƙasa
DNA da RNA da aka tsarkake ta hanyar ginshiƙin tsarkakewa, idan ion gishiri da abun ciki na furotin sun yi yawa, zai shafi gwaje-gwajen da ke ƙasa, kamar: haɓaka PCR, jujjuya rubutu, da sauransu.
1. DNA da aka eluted da RNA suna da ragowar ions gishiri.
Shawara: Tabbatar cewa an ƙara madaidaicin ƙarar cikakkar ethanol zuwa Buffer RW2, kuma a wanke ginshiƙin tsarkakewa sau biyu a saurin centrifugation da aka ƙayyade a cikin umarnin aiki;Yi centrifugation don rage gurɓatar ion gishiri.
2. DNA da RNA da aka eluted suna da ragowar ethanol.
Shawara: Bayan tabbatar da wanka tare da Buffer RW2, yi fanko bututu centrifugation a centrifugation gudun a cikin umarnin aiki;Idan har yanzu akwai ragowar ethanol, zaku iya sanya bututun da ba komai a ciki sannan ku sanya shi a cikin dakin da zafin jiki na mintuna 5 don cire ragowar ethanol zuwa mafi girma.
Jagoran Jagora:
Kwayar cuta ta DNA & RNA keɓewar Kit ɗin Umarnin
