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Menene bambance-bambance tsakanin PCR, RT-PCR, qPCR, da RT-qPCR
PCR wata hanya ce da ake amfani da ita don haɓaka DNA daga ƙaramin adadin samfurin DNA.RT-PCR tana amfani da juzu'i don samar da samfurin DNA daga tushen RNA wanda za'a iya haɓakawa.PCR da RT-PCR yawanci halayen ƙarshe ne, yayin da qPCR da RT-qPCR suna amfani da motsin ƙimar ƙimar samfur.Kara karantawa -
Gaban Gano Magunguna: Yin Nufin RNA tare da Kananan Molecules
Alurar rigakafin mRNA na Pfizer don COVID ya sabunta sha'awar amfani da ribonucleic acid (RNA) a matsayin makasudin warkewa.Koyaya, niyya RNA tare da ƙananan ƙwayoyin cuta yana da ƙalubale sosai.RNA kawai yana da tubalan gini guda huɗu: adenine (A), cytosine (C), guanine (G), da uracil (U) wanda ya maye gurbin thymin...Kara karantawa -
Menene magungunan nucleic acid?
"Magungunan Nucleic acid" suna amfani da "nucleic acid," wanda ke nufin abubuwa kamar DNA da RNA waɗanda ke sarrafa bayanan kwayoyin halitta, a matsayin magunguna.Waɗannan suna ba da damar yin niyya na ƙwayoyin cuta irin su mRNA da miRNA waɗanda ba za a iya niyya da su tare da magungunan ƙananan nauyin kwayoyin halitta da magungunan rigakafi ba, kuma akwai g...Kara karantawa -
Wasu gogewa game da siRNA a cikin RT-PCR
1. Ilimi na asali (idan kuna son ganin ɓangaren gwaji, don Allah canja wurin kai tsaye zuwa kashi na biyu) A matsayin abin da aka samo asali na PCR na al'ada, PCR na ainihi yana sa ido kan canjin adadin ƙarar samfurin a kowane zagaye na haɓakawar PCR. reaction in real time thr...Kara karantawa -
Hanyar inganta tsarin amsa gwaji na RT-PCR cikakken taƙaitaccen bayani
一, Ƙara hankali na tsarin amsawa: 1. Ware babban ingancin RNA: Nasarar cDNA kira ya fito ne daga RNA mai inganci.RNA mai inganci yakamata ya zama aƙalla ya zama cikakken tsayi kuma ba tare da masu hana masu juyar da rubutu kamar EDTA ko SDS ba.Ingancin RNA yana ƙayyade iyakar ...Kara karantawa -
Cikakken ƙirar ƙirar PCR da cikakkun bayanan PCR
Tushen ƙira na farko (ana iya magance matsalolin 99%) 1. Tsawon fari: Littafin koyarwa yana buƙatar 15-30bp, yawanci kusan 20bp.Ainihin yanayin yana da kyau ya zama 18-24bp don tabbatar da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai, amma mafi tsayi mafi kyau, tsayi mai tsayi kuma zai rage ƙayyadaddun ƙayyadaddun, da rage yawan amfanin ƙasa.2. Farko...Kara karantawa -
Tsare-tsare don qRT-PCR wanda babban Likita ya tattara cikin himma
Kowa yana magana ne game da ƙa'idar gwajin qRT-PCR, ƙirar farko, fassarar sakamako, da sauransu, amma ina tsammanin ya kamata in raba tare da ku aikin gwaji na qRT-PCR.Yana da karami, amma game da sakamako ne.Kafin yin qRT-PCR, muna buƙatar samun cikakkiyar fahimtar mu ...Kara karantawa -
Kariya don RT-qPCR
Gwajin RT-qPCR ya haɗa da cirewar RNA da ƙimar inganci, jujjuya rubutu da qPCR matakai uku, kowane mataki yana da matakan tsaro da yawa, zamu gabatar da dalla-dalla a ƙasa.Ⅰ.Ƙimar ingancin RNA A gwajin RT-qPCR, bayan an gama hakar RNA, ...Kara karantawa -
Cikakken gabatarwar zuwa ainihin lokacin PCR
1. Fahimtar farko A wannan matakin, muna buƙatar fahimtar wasu ra'ayoyi da kalmomi, don guje wa yin kuskure a gaban manyanmu, kamar: Q: Menene bambanci tsakanin RT-PCR, qPCR, PCR na ainihi, kuma RT-PCR na ainihi?Amsa: RT-PCR ita ce juyar da rubutun PCR (reverse t...Kara karantawa -
PCR, PCR da yawa, A cikin PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR
PCR, PCR da yawa, A wurin PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR Za mu warware ra'ayoyi, matakai da cikakkun bayanai na PCR daban-daban.PCR Polymerase Chain Reaction, wanda ake magana da shi azaman PCR, fasaha ce ta kwayoyin halitta wacce ake amfani da ita don haɓaka takamaiman gutsuttsuran DNA.Ana iya la'akari da ...Kara karantawa -
Ƙa'idar gwaji ta qRT-PCR
RT-qPCR an haɓaka shi daga fasahar PCR na yau da kullun.Yana ƙara sinadarai masu kyalli ( rini mai kyalli ko bincike mai kyalli) zuwa tsarin amsawar PCR na al'ada, kuma yana gano tsarin cirewa da haɓakawa na PCR a ainihin lokacin bisa ga hanyoyin haskensu daban-daban.Alamar Fluorescent...Kara karantawa -
Hanyar inganta tsarin amsa gwaji na RT-PCR cikakken taƙaitaccen bayani
Ⅰ.Ƙara hankali na tsarin amsawa: 1. Rarrabe babban ingancin RNA: Nasarar cDNA kira ya fito ne daga RNA mai inganci.RNA mai inganci yakamata ya tabbatar da aƙalla jimlar tsayi kuma baya ƙunshi masu hanawa waɗanda basu ƙunshi enzymes rikodi ba, kamar EDTA ko SDS.A qual...Kara karantawa
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