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Babban Magani Hudu Don Kula da Gurɓatar Samfuran PCR
1: Sauya kayan gwaji a cikin lokaci Saita (NTC) iko mara kyau kuma maimaita shi sau da yawa.Da zarar an gano cewa akwai gurɓataccen samfurin PCR a cikin dakin gwaje-gwaje, maye gurbin duk kayan gwaji a cikin lokaci.Kamar: sake tsarkewa da shirya abubuwan farko, sake bakara tip ɗin pipette, E ...Kara karantawa -
Biyu-dual-aiki na RT-PCR enzymes
Rubutun juzu'i na al'ada ba zai iya jure yanayin zafi mai girma ba (mafi kyawun zafin jiki don ayyukan MMLV shine 37-50°C, kuma AMV shine 42-60°C).RNA mafi hadaddun ƙwayar cuta ba za a iya jujjuya shi yadda ya kamata zuwa cDNA a ƙananan zafin jiki ba, yana haifar da ingantaccen ganowa Ragewar.Ta...Kara karantawa -
Nucleic acid isothermal haɓaka fasahar haɓakawa
PCR ita ce fasahar haɓaka haɓakar acid nucleic da aka fi amfani da ita kuma ana amfani da ita sosai saboda azanci da ƙayyadaddun sa.Koyaya, PCR yana buƙatar maimaita ƙarancin zafi kuma ba zai iya kawar da iyakokin dogaro da kayan aiki da kayan aiki ba, wanda ke iyakance aikace-aikacen sa a cikin asibiti ...Kara karantawa -
Menene rigakafin mRNA?
Menene maganin mRNA Alurar mRNA yana tura RNA zuwa sel na jiki don bayyanawa da kuma samar da antigens sunadaran bayan gyare-gyaren da suka dace a cikin vitro, ta haka ne ke jagorantar jiki don samar da amsawar rigakafi daga antigen, ta haka ne ya fadada jiki ...Kara karantawa -
PCR Technology
PCR (polymerase chain reaction) yana ɗaya daga cikin fasahar haɓaka DNA na in-vitro, tare da tarihin sama da shekaru 30.Kary Mullis na Cetus, Amurka ne ya jagoranci fasahar PCR a shekarar 1983. Mullis ya nemi takardar shaidar PCR a 1985 kuma ya buga takarda ta farko ta PCR akan Kimiyya a cikin wannan ...Kara karantawa -
Sabon gano coronavirus, daga acid nucleic, antibody zuwa antigen, ta yaya za a zaɓi daidaitawar cutar?
Annoba ta canza duniya.A duk faɗin duniya, gwamnatocin dukkan ƙasashe suna fuskantar ƙalubale masu yawa na rigakafi da shawo kan annobar.A lokacin cutar ta COVID-19, kasar Sin tana cikin matakai hudu na tsarin rigakafi da mayar da martani (rigakafi, ganowa, sarrafawa da mabuɗin don…Kara karantawa -
Hanyoyi hudu don hana gurɓatar samfuran PCR
Gina Tsarin SOP Kafa gwajin SOP na PCR don daidaita halayen ma'aikatan gwaji.Masu gwajin suna bin ƙa'idodin aiki sosai, kuma suna rage gurɓataccen gurɓataccen PCR wanda zai iya haifar da abubuwan ɗan adam ko hana faruwar ƙazanta a cikin aikin.In add...Kara karantawa -
Yadda ake haɓaka ingancin juzu'i na LncRNA?
Jagora Dogon RNA mara coding, lncRNA RNA ce mara coding mai tsayi sama da 200 nucleotides, gabaɗaya tsakanin 200-100000 nt.lncRNA yana sarrafa maganganun kwayoyin halitta a epigenetic, rubutun rubutu da matakan rubutu, kuma yana shiga cikin shiru na X chromosome, genome imprinting da chroma ...Kara karantawa -
Ana iya wucewa daga mutum zuwa mutum cikin dakika 14.Menene ya faru da nau'in Delta "mai saurin yaduwa" a cikin Zhong Nanshan Pass?
Majiyar fassara: Editan tawagar WuXi AppTec A birnin Guangzhou na kasar Sin, 'yan sandan da ke da alhakin gudanar da bincike kan cutar sun fitar da wani hoton bidiyo na sa ido: A cikin gidan abinci guda, su biyun sun shiga bandaki daya bayan daya ba tare da wani mu'amala ba.Kawai dakika 14 na haɗin gwiwa...Kara karantawa -
SNP kwayoyin lakabi da ganowa
Bayan da masanin Amurka Eric S. Lander ya gabatar da tsari guda daya na nucleotide polymorphism (SNP) a matsayin alamar kwayoyin halitta ta ƙarni na uku a cikin 1996, SNP an yi amfani da shi sosai a cikin nazarin ƙungiyar tattalin arziki, gina taswirar haɗin gwiwar kwayoyin halitta, da kuma tantance kwayar cutar ɗan adam., D...Kara karantawa -
Shahararren Kimiyya |Yadda ake gano sabon coronavirus ta qPCR
COVID-19 cuta ce mai saurin kamuwa da Cutar Cutar Cutar Coronavirus Nau'in 2. Lokacin da mutum ya kamu da cutar, alamomin da aka fi sani da su sun hada da zazzabi, tari, da ƙarancin numfashi.Ana iya tattara samfuran da aka yi amfani da su don gwaji ta hanyar swabs na nasopharyngeal ko swabs na oropharyngeal.Me zan...Kara karantawa -
Shin har yanzu kuna amfani da fasahar da aka ƙirƙira shekaru 30 da suka gabata don fitar da RNA?
Shin har yanzu kuna amfani da Trizol don cire RNA?Wallahi kar ka barni in damu da lafiyarka!Trizol yana gab da zama saniyar ware, kar a gaya mani har yanzu kuna cikin duhu?Babban bangaren Trizol reagent shine phenol, kuma ana kara reagents na halitta kamar chloroform a cikin gwajin.Kara karantawa
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