Tsarin Gina SOP

Kafa gwajin SOP na PCR don daidaita halayen ma'aikatan gwaji.

Masu gwajin suna bin ƙa'idodin aiki sosai, kuma suna rage gurɓataccen gurɓataccen PCR wanda zai iya haifar da abubuwan ɗan adam ko hana faruwar ƙazanta a cikin aikin.Bugu da ƙari, mai gwaji ya kamata ya sami daidaitattun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararru, gami da ƙwarewar aiki da kayan aikin da ke da alaƙa, fayyace duk tsarin aikin, ƙware hanyoyin jiyya na gurɓatawa da hanyoyin kula da ingancin dakin gwaje-gwaje, da samun damar fassara sakamakon gwajin daidai.

Ginin Standard PCR Laboratory

dakin gwaje-gwaje na PCR ya kasu kashi hudu bisa ka'ida, wato yankin shirye-shiryen reagent, yankin sarrafa samfurin, yankin haɓakawa, da yankin nazarin samfuran haɓakawa.Wuraren biyu na farko su ne wuraren haɓakawa, kuma yankuna biyu na ƙarshe sune wuraren haɓakawa.Ya kamata a raba yankin pre-amplification da yankin bayan haɓakawa sosai.Gwaji kayan, reagents, rikodi takarda, alkalami, tsaftacewa kayan, da dai sauransu na iya kawai gudana daga pre-amplification yankin zuwa post-amplification yankin, wato, daga reagent shirye-shirye yankin, samfurin sarrafa yankin, amplification yankin, da amplification samfurin bincike yankin, kuma dole ne ba gudãna a baya shugabanci.Hakanan ya kamata iska a cikin dakin gwaje-gwaje ta gudana daga yankin da aka riga aka haɓaka zuwa yankin bayan haɓakawa, ba a jujjuyawar ba.

Rage matakan gwaji

Idan dakin gwaje-gwaje yana yin ganowa da gano PCR kawai, ana ba da shawarar yin amfani da PCR mai kyalli maimakon PCR na al'ada.

Za'a iya tattara sakamakon gano ƙimar PCR na Fluorescence da bincika ta siginar kyalli, don haka babu buƙatar buɗe murfin don electrophoresis bayan amsawar, wanda ke guje wa gurɓatar samfuran PCR da ke haifar da zubar da samfuran dauki don samar da iska.Idan ka ƙara yawan buɗewar hula yayin ɗaukar nauyin gel electrophoresis, ana iya samun gurɓataccen iska.Ana ba da shawarar haɓaka aikace-aikacen PCR mai ƙididdigewa kuma a hankali maye gurbin PCR mai inganci.

Ɗauki tsarin hana gurɓatawa na UNG

Ana amfani da tsarin gurɓataccen samfur na anti-PCR don amsawar PCR.

Tsarin yana amfani da dUTP maimakon dTTP.Bayan amsawar PCR, duk samfuran PCR (gutsiyar DNA) an haɗa su tare da dUTP;a cikin zagaye na gaba na amsawar PCR, UNG enzyme da aka ƙara a cikin tsarin ana shigar da shi a 37 ° C na minti 5 kafin PCR, wanda zai iya zama ƙayyadaddun ƙasƙantar da duk gutsuttsuran DNA da ke dauke da dUTP, sannan a yi aikin PCR.Wannan na iya cire gaba ɗaya gurɓatar iska da samfuran PCR ke haifarwa.Ana nuna tasirin a cikin hoton da ke ƙasa:

Lura: Don jerin PCR kai tsaye, zaku iya zaɓar samfuran samfuran tsarin gurɓataccen samfuran anti-PCR na FJ Biotech

Ba da shawara

Don dakunan gwaje-gwajen da ke gudanar da gwaje-gwaje masu girma na genotyping, ana ba da shawarar sosai a yi amfani da tsarin gurɓataccen samfur na UNG anti-PCR don gwajin sakewa baya ga gina dakunan gwaje-gwaje masu ma'ana.

Tunatarwa: Amfani da wannan tsarin ba zai iya cire gurɓatar samfurin PCR da aka rigaya ya haifar ba.Don haka, yakamata a yi amfani da tsarin UNG a farkon gwajin da ya dace, kuma a koyaushe a yi amfani da tsarin UNG don haɓaka PCR, don hana gurɓatar samfuran PCR.Karya tabbatacce.

Ana ba da shawarar yin amfani da tsarin PCR-UNG kai tsaye na Forge Biotech yayin gudanar da gwaji mai girma, kamar: Plant Leaf Direct PCR Kit-UNG;

Kayan Shuka Kai tsaye PCR Kit-UNG;

Kayan Naman Dabbobi Kai tsaye PCR Kit-UNG;

Mouse Tail Direct PCR Kit-UNG;

Kifi na Zebra Direct PCR Kit-UNG.

Wannan jerin na'urori daga Foregene ba zai iya aiwatar da gano PCR cikin sauri da babban sikeli ba, har ma da hanawa da sarrafa gurɓatar samfuran PCR yadda ya kamata.