Shin har yanzu kuna amfani da Trizol don cire RNA?
Wallahi kar ka barni in damu da lafiyarka!
Trizol yana gab da zama saniyar ware, kar a gaya mani har yanzu kuna cikin duhu?

Babban bangaren Trizol reagent shine phenol, kuma ana kara abubuwan da ake amfani da su kamar chloroform a cikin aikin gwaji.Bayyanar dogon lokaci zai haifar da lahani ga lafiyar ku, musamman tuntuɓar fata kai tsaye na iya haifar da ƙona sinadarai mai tsanani da tabo na dindindin.

A karkashin yanayi na yau da kullun, abokan aikin dakin gwaje-gwaje na iya rage hatsarori ne kawai ta hanyar ba da makamai gaba daya (sanya rigar lab, safar hannu da abin rufe fuska).

Kuma shawarata a gare ku ita ce: don Allah a maye gurbin Trizol kai tsaye don guje wa cutarwa daga tushen.

1. Samfurin ƙarni na farko na hakar RNA
Fitowa a cikin 1987 (Trizol reagents)

amfani:
Karin cikakken hakar

Rashin hasara:
Ana buƙatar reagents masu guba kamar guanidine isothiocyanate-phenol-chloroform;
Aiki mai wahala;
Bukatar amfani da DNAse don cire gurɓataccen DNA

2.Na biyu ƙarni na samfurin hakar RNA
Fitowa a cikin 1994 (QIAGEN RNeasy da yawancin samfuran gida)

amfani:
Ana ɗaukar hanyar centrifugal na ginshiƙi, wanda ke da sauƙin aiki;

Hasara:
Bukatar sanya santrifuge don cire tarkacen nama
Bukatar amfani da DNAse don cire gurɓataccen DNA

3.Samfur na ƙarni na uku na hakar RNA
Yana fitowa a cikin 2005 (QIAGEN RNeasy Plus da kuma Foregenesamfuran tsarkakewa na RNA)

amfani:
Yi amfani da ginshiƙin cire DNA kai tsaye don cire DNA da tarkacen nama a lokaci guda, adana lokaci da ƙoƙari;
Amintacce kuma dacewa, babu buƙatar ƙara ginshiƙin tacewa da DNA don cire ƙazanta cikin sauƙi da gujewa gurɓatar DNA

Bari mu dubi bambance-bambancen aiki tsakanin samfurin ƙarni na farko (Trizol), samfurin ƙarni na biyu (RNeasy) da samfurin ƙarni na uku (Foregene) na hakar RNA:

Ayyukan da ke cikin adadi a bayyane yake: samfurin ƙarni na uku yana da fa'ida a bayyane

Kalmomi uku kawai don siffanta: mai sauƙi!Mai sauri!Tsaro

Taswirar PK na sakamakon gwajidominƙarni na farko, na biyu da na uku samfuran hakar RNA:

1. Electrophoretogram

Zamanin farko (1: Trizol)
Karni na biyu (2:QIAGEN RNeasy)
Karni na uku (3: QIAGEN RNeasy da, 4: Foregene)

Wani irin samfurin ne mai kyau, za ku sani ta kwatanta
RNA da aka fitar ta amfani da kayan Foregene
Ba wai kawai babban taro ba har ma da mutunci mai kyau

2.qPCR jadawali (girma mai lankwasa jadawali)

Zamanin farko (Trizol)

ƙarni na biyu (sky blueyana nufinQIAGEN RNeasy-, jayana nufinQIAGEN RNeasy +)

Ƙarni na uku (blue yana nufin Foregene, kore yana nufin QIAGEN RNeasy da ƙari)

(Lura: QIAGEN RNeasy na qPCR na ƙarni na biyu yana nufin cewa kit ɗin baya ƙara DNA kuma baya cire gurɓataccen DNA. Saboda haka, CT ya fi girma kuma sakamakon bai dace ba; QIAGEN RNeasy + yana nufin an ƙara DNA kuma an cire gurɓataccen DNA, kuma sakamakon daidai ne)

Ko electrophoretogram ne ko jadawali na ƙara girman PCR, ingancin samfuran Foregene bai yi ƙasa da samfuran da aka shigo da su waje ba.