1: Sauya kayan gwaji a cikin lokaci

Saita (NTC) iko mara kyau kuma maimaita shi sau da yawa.Da zarar an gano cewa akwai gurɓataccen samfurin PCR a cikin dakin gwaje-gwaje, maye gurbin duk kayan gwaji a cikin lokaci.Kamar: sake tsarkewa da shirya abubuwan da aka gyara, sake bakara tip pipette, EP tube, ddH2O, da sauransu, maye gurbin da sabon pipette, da aro wasu dakunan gwaje-gwaje na ɗan lokaci don yin gwajin PCR.dakin gwaje-gwajen da aka gurbata za a sami iska kuma a ba da shi tare da hasken ultraviolet har sai an kawar da gurɓataccen samfurin PCR kafin a ci gaba da gwajin.

2: Tsara lokacin bayyanar UV

Ya kamata a lura cewa don cire gurɓataccen DNA, ya kamata a tsawaita hasken ultraviolet na yau da kullun da sa'o'i 2 fiye da yadda aka saba.Duk da haka, tasirin hasken UV akan cire ƙananan guntu (kasa da 200bp) na gurɓataccen DNA har yanzu ba shi da kyau.

Tsawon igiyoyin ultraviolet (nm) gabaɗaya 254/300nm ne, kuma ya isa ya haskaka tsawon mintuna 30.Ya kamata a lura cewa lokacin zabar UV don kawar da gurɓataccen samfuran PCR, ya kamata a yi la'akari da tsawon samfurin PCR da rarraba tushe a cikin jerin samfuran.UV irradiation yana da tasiri kawai ga dogon gutsuttsura sama da 500 bp, kuma yana da ɗan tasiri akan gajerun gutsuttsura.

A lokacin hasarar UV, tushen pyrimidine a cikin samfurin PCR zai samar da dimers.Wadannan dimers na iya kawo karshen tsawo, amma ba duk pyrimidine a cikin sarkar DNA ba zasu iya samar da dimers, kuma UV irradiation zai iya karya dimers..Matsakaicin samuwar dimer ya dogara da tsawon UV, nau'in dimer na pyrimidine da jerin nucleotides da ke kusa da wurin dimer.Don haka, idan ɓangarorin da aka haɓaka na PCR ƙanana ne, ana ba da shawarar yin amfani da tsarin gurɓataccen samfur na UNG anti-PCR.

3: Abubuwan da aka saba amfani da su na gurɓacewar DNA

Aerosols ana samun sauƙin samar da su lokacin da aka ƙara pipettes, wanda ke da wuyar gujewa, kuma zai daidaita cikin sauri.Don haka, babu shakka zaɓi ne mai kyau a yawaita amfani da na'urori na musamman na gurɓatawar DNA don hana yaduwar gurɓacewar DNA.

4: Yi amfani da tsarin hana gurɓacewar muhalli na UNG

Bayan an cire gurɓataccen samfur na PCR, dakin gwaje-gwaje na iya amfani da tsarin gurɓataccen samfur na UNG anti-PCR don hana gurɓatar samfurin PCR.A cikin dakunan gwaje-gwaje gabaɗaya, zaku iya aiwatar da sassauƙan ɓangarorin gwaji, keɓance yankin samfur na PCR da sauran wurare, kafa wasu ƙa'idodi da ƙa'idodi na dakin gwaje-gwaje, da gudanar da horon da suka dace don hana faruwar gurɓataccen samfur na PCR.

Shawarwari: Ƙirƙirar dakin gwaje-gwaje na PCR mai ma'ana, kiyaye kyakkyawan yanayin PCR, ƙirƙira daidaitattun hanyoyin aiki na PCR, da haɓaka wayar da kan masu gwaji su ne maɓallai don hanawa ko rage gurɓatar gwajin PCR.