Kayan shuka kai tsaye PCR kit-UNG (ba tare da Kayan Samfura ba) Yarjejeniya kai tsaye PCR daga Kayan Shuka
Bayanin Kit:
Yi amfani da leaf ko leaf lysate kai tsaye azaman samfuri don haɓakawa na PCR, ba tare da ware DNA daban ba, sauri da hankali sosai.An kara tsarin hana gurbatar yanayi na UNG don hana abubuwan da ba su dace ba.
◮Babu tsarkakewar DNA mai cin lokaci da tsada (Lokaci, aiki da ƙimar kuɗi!)
◮Ƙananan abu
◮Simple-samfurin za a iya hõre PCR dauki ko lysis dauki ba tare da yankan ko nika
◮2 X Mix-rage samfurin loading kurakurai da dauki tsarin lokaci shirye-shiryen
◮Ana iya kammala shirye-shiryen samfuri mai sauri a cikin mintuna 10, ana iya kammala aikin PCR a cikin mintuna 50
◮Babban kayan aiki-ana iya kammala amsawar lysis a cikin farantin PCR-96 mai kyau
Bayani
Dangane da kayan aikin PCR na Leaf Direct, ana amfani da dUTP maimakon dTTP, kuma ana ƙara UNG enzyme wanda zai iya lalata samfurin da ke ɗauke da dUTP.Ta wannan hanyar, ana tabbatar da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun haɓakawa, kuma ana hana matsalar gurɓacewar samfuran PCR da ka iya faruwa yayin gwajin ƙwayoyin cuta masu girma.
Ƙayyadaddun bayanai
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Abubuwan Kit
| Kashi na I | Buffer P1 |
| Buffer P2 | |
| 6×Mai Buffer Loading DNA | |
| Kashi na II | 2× Leaf PCR EasyTMMix(UNG) |
Fasaloli & fa'idodi
Babu tsarkakewar DNA mai cin lokaci da tsada
■ Ƙananan abu
∎ Sauƙaƙe-samfurin za a iya jujjuya shi zuwa amsawar PCR ko amsawar lysis ba tare da yanke ko niƙa ba
∎ 2 × Mix, rage kuskuren ɗorawa samfurin da lokacin shirye-shiryen tsarin amsawa
∎ Za a iya kammala shirye-shiryen samfuri cikin sauri cikin mintuna 10, ana iya kammala aikin PCR cikin aƙalla mintuna 50.
∎ Za'a iya kammala amsawar ɗimbin kayan aiki mai girma a cikin farantin PCR mai rijiyar 96
Kayan aiki sigogi
Aikace-aikace: ganewar asali da aka gyara, ƙirar genotyping, da sauransu.
Misali: ganyen shuka
Sashi: Diamita 2-3mm (hanyar kai tsaye), diamita 5-7mm (hanyar lysis)
Kewayon ganowa: guntun manufa ≤1kb
Gudun aiki
Electropherogram
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