Tsarin Gano DNA na Foregene Y Plus (Rashin Haɓakawa)
Bayanin Kit:
ForegeneTsarin gano DNA Y Plus yana amfani da fasaha mai launi mai launi shida don haɓaka 20 Y chromosome core loci, 15 fi so loci, 6 madadin loci da 3 Y indel loci lokaci guda..
Bayani
ForegeneTsarin gano DNA Y Plus yana amfani da fasaha mai launi mai launi shida don haɓaka 20 Y chromosome core loci, 15 fi so loci, 6 madadin loci da 3 Y indel loci lokaci guda..
Abun ciki Kit
Shirye-shiryen tsarin haɓakawa
Theabun da ke ciki na shirina standard(ba tare da cirewa ba)tsarin haɓakawa
| Cmasu kai hari | 25 μltsarin (μl) | 10 μl tsarin (μl) | |
| Babban Mix | 4 × Jagora MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Deionized ruwa | 13.75 | 5.5 | |
| Bruwa tabo | Diamita 1.2mm | Diamita 1.2mm | |
| Total dauki girma | 25 μl | 10 μl | |
Binciken Bayanai
Haɗe-haɗe Graph 1:Taswirar Buga Tsani na Allelic
Haɗe-haɗe Graph 2:Buga DNA misali 9948 bugawataswira
Ma'ajiyar Reagent
1. Da fatan za a adana a ƙasa -20 ° C (Idan ba a yi amfani da shi na ɗan lokaci ba), bayan karɓar kayan daskararre a cikin busassun kankara ko fakitin kankara.
2. Da fatan za a adana ɓangaren pre-reaction 4 × premix VII a -20 ° C, bayan an fitar da kit ɗin don amfani, kuma sauran sauran abubuwan da suka rage an adana su a 4 ° C don guje wa daskarewa da narke maimaitawa.Idan kashi ɗaya ne ƙananan, ana bada shawara don adanawa a -20 ° C bayan an yi amfani da shi.
3. Ajiye abubuwan da aka gyara bayan amsawa a 4 ° C, kauce wa daskarewa da narke maimaitawa, kuma kada ku taɓa reagents kafin amsawa don guje wa gurɓatawa.
