Tsarin Gano DNA na Foregene 27Y
Bayanin Kit:
Foregene Tsarin Ganewar DNA 27Y yana amfani da fasaha mai alamar kyalli mai launi shida don haɓaka 27 Y-STR loci a lokaci ɗaya.Ciki har da: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYF387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Kit ɗin yana da kyakkyawan ikon hana hanawa, kuma yana iya haɓaka takarda kai tsaye, katin FTA, swab na auduga, katin saliva, swab na baka, kuma ana iya amfani dashi don cirewa da tsarkake samfuran DNA daga kayan harka.
Bayani
Foregene Tsarin Ganewar DNA 27Y yana amfani da fasaha mai alamar kyalli mai launi shida don haɓaka 27 Y-STR loci a lokaci ɗaya.Ciki har da: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYF387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Kit ɗin yana da kyakkyawan ikon hana hanawa, kuma yana iya haɓaka takarda kai tsaye, katin FTA, swab na auduga, katin saliva, swab na baka, kuma ana iya amfani dashi don cirewa da tsarkake samfuran DNA daga kayan harka.
Abun ciki Kit
| Kayan gwajin Reagent | Sunan sashi | girma (μl/tube) | Yawan (tube) |
| PCR pre-reaction kit | 2 × PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Babban Mix | 500 | 2 | |
| DNA Buga Standard 9948 | 20 | 2 | |
| Deionized ruwa | 1700 | 2 | |
| Kit ɗin amsawa bayan PCR | 27Y allele cakuda Tsani | 20 | 2 |
| Ma'aunin nauyin kwayoyin Orange na ciki ORG500 | 150 | 2 |
Binciken Bayanai
1. Buɗe Gene Mapper ID software.Lokacin amfani da wannan kit a karon farko, kuna buƙatar shigo da bangarori, layin girman rubutu (ORG500: 450, 420, 490, 5000, 500, 500, 500, 500, 500).
2. Shigo da bayanan electrophoresis, zaɓi sigogin bincike masu dacewa kamar panel, hanyar bincike, da daidaitattun girman, kuma canza nau'in samfurin Tsani zuwa "Allelic Ladder" a cikin "Sample Type" shafi;fara nazarin bayanan.
Ma'ajiyar Reagent
1. Da fatan za a adana su a ƙasa -20 ° C (idan ba a yi amfani da su na ɗan lokaci ba), bayan karɓar kayan aikin daskararre a busassun kankara ko gel ɗin kankara.
2. Da fatan za a adana kit ɗin a 4 ° C kafin amsawa don guje wa daskarewa da narke maimaitawa, bayan an fitar da kayan don amfani,;Ya kamata a adana enzyme a -20 ° C.
2. Bayan amsawa, kit ɗin (ciki har da ma'aunin ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta) ya kamata a adana shi a cikin "dakin ganowa na electrophoresis" a 4 ° C, kauce wa daskarewa da narke mai maimaitawa, da kuma guje wa haɗuwa da kayan aikin da aka rigaya don kauce wa gurɓatawa.
