Tsarin Gano DNA na Foregene 25A-C (Case)
Bayanin Kit:
Foregene DNA Identification System 25A-C yana amfani da fasaha mai launi mai launi shida don haɓaka 23 autosomal STR loci, Amel loci ɗaya, Yindel loci guda ɗaya da loci mai inganci na ciki guda biyu a lokaci guda don haɓaka samfuran DNA da aka cire kuma aka tsarkake daga samfuran harka.
Bayani
Foregene DNA Identification System 25A-C yana amfani da fasaha mai launi mai launi shida don haɓaka 23 autosomal STR loci, Amel loci ɗaya, Yindel loci guda ɗaya da loci mai inganci na ciki guda biyu a lokaci guda don haɓaka samfuran DNA da aka cire kuma aka tsarkake daga samfuran harka.
Abun ciki Kit
Shirye-shiryen tsarin haɓakawa
Haɗin Shiri na daidaitaccen tsarin hakar da haɓakawa
| Abubuwan da aka gyara | 10 μl tsarin (μl) | |
| Babban Mix | 2.5 × PCR Premix C Ⅰ | 4.0 |
| 10×25A-CPrimer Mix | 1.0 | |
| Samfurin DNA | 1-5 | |
| Deionized ruwa | Yi har zuwa ƙarar amsawa na 10μl |
Binciken Bayanai
Haɗe-haɗe Graph 1:Tsani genotyping na allele
Haɗe-haɗe Graph 2:Buga DNA misali 9948 bugawataswira
Ma'ajiyar Reagent
1. Bayan karbar kit, da fatan za a adana shi a ƙasa -20 ℃ idan ba ku yi amfani da shi ba don lokacin.
2. Bayan da kit da aka dauka fitar da amfani, da pre-reaction bangaren PCR premixed bayani da aka adana a -20 ℃, da kuma sauran pre-reaction reagents ana adana a 4 ℃ don kauce wa maimaita daskare-narke.Idan kashi ɗaya yana ƙarami, ana bada shawara don adanawa a -20 ° C bayan marufi.
3. Bayan da dauki, da aka gyara ana kiyaye a 4 ℃ don kauce wa maimaita daskare-narke.Kar a taɓa reagents pre-reaction don guje wa gurɓatawa.
