Ba wai kawai za mu gwada mafi girman mu don ba ku samfuran samfuran da sabis ga kowane mai siye ɗaya ba, amma kuma suna shirye don karɓar duk wani shawarwarin da masu siyanmu suka bayar don Manufacturer na Nucleic Acid Purification Rna Isolation DNA Extraction Reagent Kit a cikin 96-Well Plate, Kamfaninmu yana kula da kasuwancin aminci gauraye da gaskiya da gaskiya don ci gaba da dogon lokaci dangantaka da abokan cinikinmu.
Ba wai kawai za mu gwada mafi girman mu don ba ku fitattun samfura da ayyuka ga kowane mai siye ɗaya ba, har ma a shirye muke mu karɓi kowace shawara da masu siyan mu suka bayar donKit ɗin Haɗin Acid Nucleic Acid na China da Gano PCR, Kamfaninmu yana shayar da sababbin ra'ayoyi, kula da ingancin inganci, cikakken kewayon sabis na bin diddigin, da kuma bi don yin samfuran inganci.Kasuwancinmu yana nufin "masu gaskiya da aminci, farashi mai kyau, abokin ciniki na farko", don haka mun sami amincewar yawancin abokan ciniki!Idan kuna sha'awar kayanmu da sabis ɗinmu, don Allah kar a yi shakka a tuntuɓe mu!

Bayani

Wannan kit ɗin yana amfani da ginshiƙin jujjuyawar da dabarar da Foregene ya haɓaka, wanda zai iya fitar da tsafta mai inganci da ingancin jimlar RNA daga sel waɗanda aka kirkira a cikin faranti 96, 24, 12, da 6-riji.

Kit ɗin yana ba da ingantacciyar ginshiƙin Tsabtace DNA, wanda zai iya raba mai ƙarfi da sel lysate cikin sauƙi, ɗaure da cire DNA na genomic.Aikin yana da sauƙi kuma yana adana lokaci.

Rukunin RNA-kawai na iya ɗaure RNA da kyau da kyau tare da tsari na musamman.Ana iya sarrafa adadi mai yawa na samfurori lokaci guda.

Abubuwan Kit

Da fatan za a sa safar hannu kuma ku ɗauki matakan kariya yayin aikin kamar yadda Buffer cRL1 da Buffer RW1 ke ɗauke da gishiri masu ban haushi.

Fasaloli & fa'idodi

∎ Dukkanin tsarin ana sarrafa shi a cikin zafin jiki (15-25 ℃), ba tare da wankan kankara da ƙananan zafin jiki ba.
∎ Gabaɗaya kit ɗin ba shi da RNase, babu buƙatar damuwa game da lalata RNA.
■ Rukunin Tsabtace DNA yana ɗaure DNA ta musamman, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara ƙarin DNA ba.
∎ Yawan yawan amfanin RNA: Rukunin RNA-kawai da dabara na musamman na iya tsarkake RNA da kyau.
n Gudun sauri: mai sauƙin aiki kuma ana iya kammala shi cikin mintuna 11.
∎ Tsaro: Ba a buƙatar reagents na halitta.
∎ Kyakkyawan inganci: RNA da aka tsarkake tana da tsafta, ba ta da furotin da sauran ƙazanta, kuma tana iya saduwa da gwaje-gwaje daban-daban na gaba.

Kit aikace-aikace

Ya dace da hakar da tsarkakewa na jimlar RNA daga sel masu al'ada a cikin faranti 96, 24, 12, da 6-rijiya.

Gudun aiki

zane

Zane-zanen baturi na Agarose Gel na Kit ɗin Warewa na Cell Total RNA ya yi maganin lambobi daban-daban na sel, 20μl elution, ɗauki 2μl tsarkakakkun jimlar RNA 1%.

Adana da rayuwar shiryayye

Ana iya adana kit ɗin na tsawon watanni 12 a zazzabi na ɗaki (15-25 ℃) ko 2-8 ℃ na tsawon lokaci (watanni 24).

Buffer cRL1 za a iya adana a 4 ℃ ga 1 wata bayan ƙara 2-hydroxy-1-ethanethiol (na zaɓi) .We ba kawai za mu yi kokarin mu mafi girma don bayar da ku fice samfurori da kuma ayyuka ga kowane guda mai siye, amma kuma a shirye su karbi duk wani shawara bayar da mu buyers ga Manufacturer na Nucleic Acid tsarkakewa Rna warewa kamfanin mu aminci gauraye da sabis na DNA, mu gauraye gauraye da kayayyakin da sabis, da aminci ga kowane mai siye. don ci gaba da dogon lokaci dangantaka da abokan cinikinmu.
Maƙerin naKit ɗin Haɗin Acid Nucleic Acid na China da Gano PCR, Kamfaninmu yana shayar da sababbin ra'ayoyi, kula da ingancin inganci, cikakken kewayon sabis na bin diddigin, da kuma bi don yin samfuran inganci.Kasuwancinmu yana nufin "masu gaskiya da aminci, farashi mai kyau, abokin ciniki na farko", don haka mun sami amincewar yawancin abokan ciniki!Idan kuna sha'awar kayanmu da sabis ɗinmu, don Allah kar a yi shakka a tuntuɓe mu!

Ba a fitar da RNA ko amfanin RNA yayi ƙasa

Sau da yawa akwai dalilai iri-iri waɗanda ke shafar ingancin farfadowa, kamar: samfurin nama na RNA abun ciki, hanyar aiki, ƙarar haɓaka, da sauransu.

1. Ice wanka ko cryogenic (4 ° C) centrifugation aka yi a lokacin aiki.

Shawarwari: Yi aiki a dakin da zafin jiki (15-25 ° C) a duk tsawon tsari, kada ku yi wanka da centrifuge a ƙananan yanayin zafi.

2. Ƙimar samfurin da ba daidai ba ko yawan lokacin ajiyar samfurin.

Shawarwari: Ajiye samfurori a -80 ° C ko daskare a cikin ruwa nitrogen kuma kauce wa daskare-narke maimaita amfani;yi ƙoƙarin amfani da sabobin nama ko ƙwayoyin al'ada don hakar RNA.

3. Rashin isasshen samfurin lysis.

Shawarwari: Lokacin da aka haɗa nama, tabbatar da cewa nama ya isa daidai kuma cewa ƙwayoyin nama sun rabu sosai don bayyana sakin RNA.

4. Ba a ƙara eluent daidai ba.

Shawarwari: Tabbatar da cewa RNase-Free ddH₂O ana ƙara juzu'i zuwa tsakiyar membrane ginshiƙin tsarkakewa.

5. Ba a ƙara madaidaicin ƙarar cikakken ethanol zuwa Buffer RL2 ko Buffer RW2 ba.

Shawarwari: Bi umarnin, ƙara madaidaicin ƙarar cikakkar ethanol zuwa Buffer RL2 da Buffer RW2 kuma gauraya da kyau kafin amfani da kit ɗin.

6. Nama samfurin sashi bai dace ba.

Shawarwari: Yi amfani da 10-20 MG na nama ko (1-5) × 10⁶Kwayoyin a cikin 500 μl buffer RL1, saboda yawan amfani da nama zai iya haifar da raguwar hakar RNA.

7. Rashin ingantaccen ƙarar ƙyalli ko rashin cikawa.

Shawarwari: Girman haɓakar ginshiƙin tsarkakewa shine 50-200 μl;idan tasirin elution bai gamsar ba, ana ba da shawarar tsawaita lokacin sanyawa dakin zafin jiki bayan ƙara preheated RNase-Free ddH₂O, misali na 5-10 min.

8.Shafin tsarkakewa yana da ragowar ethanol bayan wankewar buffer RW2.

Shawarwari: Idan akwai ragowar ethanol bayan wankewar Buffer RW2, centrifugation mara amfani na 1min, lokacin aikin centrifugation na bututu mara kyau za'a iya ƙara zuwa 2min, ko kuma ana iya sanya ginshiƙin tsarkakewa a dakin da zafin jiki na 5 min don cire isasshen ethanol.

RNA mai tsafta ya lalace

Ingancin RNA da aka tsarkake yana da alaƙa da abubuwa kamar adana samfurin, gurɓataccen RNase, da magudi, da sauransu.

1. Ba a adana samfuran nama a cikin lokaci.

Shawarwari: Idan ba a yi amfani da samfurori na nama ko sel a cikin lokaci ba bayan tattarawa, nan da nan a adana a -80 ° C ko nitrogen mai ruwa.Don cire RNA, yi amfani da sabon samfurin nama ko tantanin halitta a duk lokacin da zai yiwu.

2. Maimaita daskare-narke samfuran nama.

Shawarwari: Lokacin adana samfuran nama, yana da kyau a yanke su kanana don adanawa, kuma a cire ɗaya daga cikin guntun lokacin amfani da su don guje wa daskare-narke samfurin da kuma lalata RNA.

3. Ana gabatar da RNase ko a'a sanye da safar hannu, abin rufe fuska, da sauransu yayin aikin.

Shawarwari: Gwajin cirewar RNA an fi yin su a cikin ɗakunan sarrafa RNA daban kuma an share teburin kafin gwajin.

Sanya safar hannu da abin rufe fuska yayin gwajin don rage lalata RNA wanda ya haifar da gabatarwar RNase.

4. Reagents sun gurbata da RNase yayin amfani.

Shawarwari: Sauya da sabon Kit ɗin Keɓewar Dabbobi na RNA don gwaje-gwaje masu alaƙa.

5. Bututun centrifuge, tukwici, da sauransu da ake amfani da su wajen sarrafa RNA sun gurɓata da RNase.

Shawarwari: Tabbatar da cewa bututun centrifuge, tukwici, pipettes, da sauransu. da ake amfani da su wajen cire RNA duk ba su da RNase.

RNA da aka tsarkake yana shafar gwaje-gwaje na ƙasa

RNA da aka tsarkake ta hanyar ginshiƙin tsarkakewa, idan ions gishiri, abun cikin furotin ya yi girma zai shafi gwajin ƙasa, kamar: juzu'i na baya, Northern Blot et al.

1. RNA da aka elutioned yana da ragowar ion gishiri.

Shawarwari: Tabbatar da cewa an ƙara madaidaicin ƙarar ethanol zuwa Buffer RW2 kuma kuyi ginshiƙan tsarkakewa guda 2 a saurin centrifugal da aka nuna don aiki;idan akwai ragowar ion gishiri, bar ginshiƙin tsarkakewa zuwa Buffer RW2 na 5 min a zafin daki kuma yi centrifugation don haɓaka kawar da gurɓataccen gishiri.

2. Ethanol saura a cikin elutioned RNA.

Shawarwari: Tabbatar da cewa bayan buffer RW2 wanka, yi aikin centrifugation mara amfani a cikin saurin centrifugation da aka nuna don aiki, ƙara lokacin aikin centrifugation mara amfani zuwa 2 min idan har yanzu akwai sauran ragowar ethanol, ko barin shi a cikin zafin jiki na 5 m.