Tsarin Gano DNA na Foregene 30 AC (Case)
Bayanin Kit:
Foregene Tsarin gano DNA30A-C yana amfani da fasaha mai launi mai launi shida dongirma 29autosomalSTR loci, 1 Amel locus, 1 Yindelwuri, kuma1 Shafukan Jima'i na musamman nau'in ɗan adam, 2wuraren kula da inganci na ciki, ana amfani da su don haɓakaDNASamfurin cirewa da tsarkakewa dagaCkayan abu.
Bayani
Foregene Tsarin gano DNA30A-C yana amfani da fasaha mai launi mai launi shida dongirma 29autosomalSTR loci, 1 Amel locus, 1 Yindelwuri, kuma1 Shafukan Jima'i na musamman nau'in ɗan adam, 2wuraren kula da inganci na ciki, ana amfani da su don haɓakaDNASamfurin cirewa da tsarkakewa dagaCkayan abu.
Abun ciki Kit
Shirye-shiryen tsarin haɓakawa
Tebura 2: Tsarin Shirye-shiryen Haɗin Daidaitaccen Tsarin hakar da haɓakawa
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| Cmasu kai hari | 25ul tsarin (µl) | 10 μl tsarin (μl) |
| Babban Mix | 2.5× PremixCⅡ | 10.0 | 4.0 |
| 5 x 30 AC Primer Mix | 5.0 | 2.0 | |
| Samfurin DNA | 2.5-10 | 1-4 | |
| Deionized ruwa | Yi har zuwa ƙarar martani na 25μl | Yi har zuwa ƙarar martani na10 μl ku |
Binciken Bayanai
Haɗe-haɗe Graph 1:Taswirar Buga Tsani na Allelic
Haɗe-haɗe Graph 2:Buga DNA misali 9948 bugawataswira
Ma'ajiyar Reagent
1. Da fatan za a adana a ƙasa -20 ° C,, bayan an karɓi kayan daskararre a busassun kankara ko fakitin kankara.
2. Da fatan za a adana ɓangaren pre-reaction 2.5 × premix CII a -20 ° C bayan an fitar da kit ɗin don amfani, kuma ana adana sauran abubuwan da suka rage kafin amsawa a 4 ° C don guje wa daskarewa da narke maimaitawa.Idan kashi ɗaya ne ƙananan, ana bada shawara don adanawa a -20 ° C bayan an yi amfani da shi.
3. Ajiye abubuwan da aka gyara bayan amsawa a 4 ° C, kauce wa daskarewa da narke maimaitawa, kuma kada ku taɓa reagents kafin amsawa don guje wa gurɓatawa.
