Jini RNA keɓewar Kit
Bayanin Kit:
Cat. No.RE-04011/04013
Domin jimlar tsarkakewar RNA daga jini gaba ɗaya 104 ≤ Farin Kwayoyin Jini ≤ 107
Da sauri ware da tsarkake jini RNA daga farin jini Kwayoyin.
-Babu buƙatar damuwa game da lalata RNA.Gaba dayan kayan aikin ba shi da RNase
-Mai sauƙi-dukkan ayyukan ana kammala su a cikin zafin jiki
-Fast — ana iya kammala aikin a cikin mintuna 20
-Babban yawan amfanin RNA: Rukunin RNA-kawai da tsari na musamman na iya tsarkake RNA da kyau
-Safe-ba a yi amfani da reagent Organic ba
-Babban ƙarfin sarrafa samfurin-har zuwa samfuran 200μl ana iya sarrafa su kowane lokaci.
-Maɗaukaki mai inganci— RNA mai tsafta yana da tsafta sosai, ba shi da furotin da sauran ƙazanta, kuma yana iya saduwa da aikace-aikacen gwaji iri-iri na ƙasa.
Bayani
Kit ɗin yana ɗaukar ginshiƙi na juzu'i da dabarar da kamfaninmu ya haɓaka, wanda zai iya fitar da inganci mai inganci da ingantaccen jimlar RNA daga gabaɗayan jinin da ke hana ruwa gudu.Kit ɗin yana ba da lysate na jan jini (Buffer RCL), wanda zai iya saurin lalata ƙwayoyin jinin ja da kuma riƙe farin jini.Ingantacciyar DNA-Cleaning Colunm na iya raba abubuwan da ke sama da sel lysates cikin sauƙi da haɓakawa da cire DNA na genomic.Aikin yana da sauƙi kuma yana adana lokaci;Rukunin RNA-kawai na iya ɗaure RNA da kyau sosai, kuma tare da tsari na musamman, yana iya aiwatar da adadi mai yawa na samfurori a lokaci guda.
Duk tsarin RNase-Free yana sa RNA da aka fitar ba ta lalacewa;Buffer RW1 da tsarin wanke buffer RW2 yana sanya RNA da aka samu kyauta daga furotin, DNA, ions da gurɓataccen fili.
Abubuwan da ke cikin Kit
| Jimlar Jini Kit ɗin Warewa RNA | ||
| Kayan abun ciki | RE-04011 | RE-04013 |
| sau 50 | sau 200 | |
| Matsakaicin RCL (10×) | 52.5 ml | ml 210 |
| Buffer BRL1* | ml 30 | 120 ml |
| Farashin BRL2 | ml 18 | ml 66 |
| RW1* | ml 25 | 100 ml |
| Farashin RW2 | 24ml ku | ml 96 |
| RNase-Free ddH2 O | ml 10 | ml 40 |
| Rukunin RNA-kawai | 50 sets | 200 sets |
| Rukunin Tsabtace DNA | 50 sets | 200 sets |
| manual | 1 kwafi | 1 kwafi |
Fasaloli & fa'idodi
-Babu buƙatar damuwa game da lalata RNA.Duk kayan aikin ba shi da RNase.
-Mai sauƙi-dukkan ayyukan ana kammala su a cikin zafin jiki.
-Fast — ana iya kammala aikin a cikin mintuna 20.
-Babban yawan amfanin RNA: Rukunin RNA-kawai da tsari na musamman na iya tsarkake RNA da kyau.
-Safe-ba a yi amfani da reagent Organic ba.
-Babban ƙarfin sarrafa samfurin-har zuwa samfuran 200μl ana iya sarrafa su kowane lokaci.
-Maɗaukaki mai inganci— RNA mai tsafta yana da tsafta sosai, ba shi da furotin da sauran ƙazanta, kuma yana iya saduwa da aikace-aikacen gwaji iri-iri na ƙasa.
Kayan aiki sigogi
Aikace-aikacen Kit:
Ya dace da cirewa da tsarkakewar jimillar RNA daga jinin dabbobi masu shayarwa.
Gudun aiki
Yanayin ajiya
Buffer RCL (10 ×) ya kamata a adana shi a 2-8 ℃;sauran abubuwan da aka gyara na kit za a iya adana su a dakin da zafin jiki (15-25 ℃) a karkashin bushe yanayi, kuma za a iya adana shi har tsawon watanni 12.Ana iya adana buffer BRL1 a 4 ℃ na wata 1 bayan ƙara β-mercaptoethanol (na zaɓi) .
Lura: Idan an adana shi a ƙananan zafin jiki, maganin yana da sauƙi ga hazo.Tabbatar sanya maganin a cikin kit a cikin zafin jiki na wani lokaci kafin amfani.Idan ya cancanta, preheta shi a cikin ruwan wanka na 37 ° C na tsawon minti 10 don narkar da hazo, kuma a gauraya da kyau kafin amfani.
Jagora don nazarin matsalolin
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Ba za a iya fitar da RNA ba ko yawan amfanin acid nucleic ya yi ƙasa
Yawancin lokaci akwai dalilai da yawa waɗanda ke shafar ingancin farfadowa, kamar: samfurin abun ciki na RNA, hanyar aiki, ƙarar haɓakawa, da sauransu.
Binciken dalilai na gama gari:
1.Ice wanka ko ƙananan zafin jiki (4 ° C) centrifugation yayin aiki.
Shawarwari: Yanayin zafin jiki (15-25 ° C) aiki, ba wankan kankara da ƙananan zafin jiki ba.
2. Samfurin da ba daidai ba ko ajiyar samfurin na dogon lokaci.
Shawara: Ajiye samfurori a -80 ° C ko daskare a cikin ruwa nitrogen, kuma kauce wa daskare-narke maimaita amfani;gwada amfani da sabbin samfuran da aka tattara don hakar RNA.
3. Rashin isasshen samfurin lysis
Shawarwari: Da fatan za a tabbatar da cewa samfurin da maganin aiki (Linear Acrylamide) an haɗa su sosai kuma an sanya su na minti 10 a zazzabi na ɗaki (15-25 ° C)
4.An ƙara eluent ba daidai ba
Shawarwari: Tabbatar cewa an ƙara ddH2O-Free RNase zuwa tsakiyar membrane na ginshiƙin tsarkakewa.
5.Ba daidai ba girma na anhydrous ethanol a cikin Buffer viRW2
Shawara: Da fatan za a bi umarnin, ƙara madaidaicin ƙarar ethanol mai anhydrous zuwa Buffer viRW2 kuma a haɗa su da kyau kafin amfani da kit ɗin.
6.Yin amfani da samfur mara kyau.
Shawara: 200µl na samfurin a kowace 500μl na Buffer viRL.Ƙarfin samfurin da ya wuce kima zai haifar da raguwar adadin hakar RNA.
7.Ba daidai ba ƙarar ƙira ko rashin cikawa.
Shawarwari: Ƙaƙwalwar haɓakar ginshiƙin tsarkakewa shine 30-50μl;idan tasirin elution bai gamsar ba, ana ba da shawarar ƙara ddH mai zafi-Free RNase.2O kuma ƙara lokacin sanyawa a zafin jiki, kamar minti 5-10
8.Purification shafi yana da ragowar ethanol bayan kurkura a cikin Buffer viRW2.
Shawara: Idan har yanzu ethanol ya kasance bayan kurkura a cikin Buffer viRW2 da fanko-tube centrifugation na 2min, za'a iya barin ginshiƙin tsarkakewa a cikin zafin jiki na 5min bayan centrifugation na bututu don cikakken cire sauran ethanol.
Lalacewar ƙwayoyin RNA masu tsafta
Ingancin RNA da aka tsarkake yana da alaƙa da abubuwa kamar ajiyar samfur, gurɓataccen RNase, da aiki.
Binciken dalilai na gama gari:
1.Ba a adana samfurori da aka tattara a lokaci ba.
Shawara: Idan ba a yi amfani da samfurin a cikin lokaci bayan tattarawa ba, da fatan za a adana shi a -80 ℃ ko nitrogen ruwa nan da nan.Don hakar kwayoyin RNA, gwada amfani da samfurori da aka tattara a duk lokacin da zai yiwu.
2. Samfuran da aka tattara suna daskarewa kuma suna narke akai-akai.
Shawara: A guji maimaita daskarewa da narke (ba fiye da sau ɗaya ba) yayin tattara samfuri da adanawa, in ba haka ba yawan adadin acid nucleic zai ragu.
An gabatar da 3.RNase a cikin dakin aiki ko kuma ba a sa safar hannu, abin rufe fuska, da sauransu.
Shawara: An fi yin hakar gwajin kwayoyin RNA a cikin wani dakin aikin RNA daban, kuma ana tsaftace teburin gwajin kafin gwajin.Saka safofin hannu da abin rufe fuska yayin gwajin don guje wa lalatawar RNA ta hanyar gabatarwar RNase.
4.The reagent yana gurbata ta RNase yayin amfani.
Shawara: Sauya da sabon Katin Keɓewar RNA na ƙwayar cuta don gwaje-gwaje masu alaƙa.
5.Cibiyar RNase na bututun centrifuge, tukwici na pipette, da sauransu. Shawara: Tabbatar cewa bututun centrifuge, tukwici na pipette, da pipettes duk ba su da RNase.
Kwayoyin RNA da aka tsarkake sun shafi gwaje-gwaje na ƙasa
Kwayoyin RNA da aka tsarkake ta hanyar ginshiƙin tsarkakewa za su yi tasiri ga gwaje-gwajen da ke ƙasa idan akwai ions gishiri ko sunadaran da yawa, kamar: fassarar juzu'i, Northern Blot, da sauransu.
1.Akwai sauran ions gishiri a cikin kwayoyin RNA da aka eluted.
Shawarwari: Tabbatar cewa an ƙara madaidaicin ƙarar ethanol anhydrous zuwa Buffer viRW2, kuma ku wanke ginshiƙin tsarkakewa sau biyu bisa ga madaidaicin saurin centrifugation akan umarnin aiki;Idan har yanzu akwai sauran ions gishiri, zaku iya ƙara Buffer viRW2 zuwa ginshiƙin tsarkakewa, kuma ku bar shi a cikin dakin zafin jiki na 5min.Sa'an nan kuma yi centrifugation don cire gurɓatar ions gishiri zuwa mafi girma
2.Akwai sauran ethanol a cikin kwayoyin RNA da aka eluted
Shawara: da zarar an tabbatar da cewa Buffer viRW2 ya wanke ginshiƙan tsarkakewa, aiwatar da centrifugation na fanko bisa ga saurin centrifugal akan umarnin aiki.Idan har yanzu akwai sauran ethanol, ana iya barin shi na mintuna 5 a zafin jiki na ɗaki bayan centrifugation na bututu don cire sauran ethanol zuwa mafi girma.
Jagoran Jagora:
Littafin Umarnin Keɓewa na RNA Viral
