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Tsarin Tsabtace PCR PCR Tsabtace Tsabtace Don DNA mai tsafta
Tsarkakewa da sauri da samun ɓangarorin DNA masu inganci daga tsarin PCR;Idan kuna son rabuwa da samun takamaiman gutsuttsuran DNA, da fatan za a zaɓi kayan dawo da gel.
◮ Faɗin farfadowar DNA: Ana iya dawo da gutsuttssun DNA masu gajeru kamar 60bp kuma masu girma kamar 10kb.
◮ Ingantaccen farfadowa: A dawo da inganci gabaɗaya fiye da 80%, kuma mafi girma zai iya kaiwa fiye da 95%.
◮ saurin gudu:Sauƙi don aiki, ana iya kammala dawo da guntun DNA a cikin mintuna 15 kuma ana iya cire dimer ɗin kai tsaye ba tare da dawo da gel ba.
◮ Sfety: Babu Organic reagent hakar da ake bukata.
◮ Kyakkyawan inganci: Gutsutsayen DNA da aka kwato suna da tsabta sosai, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
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Na'urar Warewar DNA Bacterial Na'urar Tsabtace Tsabtace Bacterial Genomic DNA
Da sauri tsarkake DNA mai inganci mai inganci daga ƙwayoyin cuta a cikin lokacin haɓaka logarithmic.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
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Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits daga Mouse Tail
Kit ɗin mafi sauri a duniya don cire DNA genomic jet ɗin linzamin kwamfuta, wanda zai iya fitar da DNA mai inganci mai inganci daga jelar linzamin kwamfuta a cikin sa'a 1.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
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Kit ɗin Cirar Gel na DNA Gel
Da sauri da kuma yadda ya kamata mai da 20bp-10kb DNA gutsuttsura daga agarose gel.
◮Faɗin farfadowa na DNA:Ana iya dawo da gutsuttsuran DNA gajarta kamar 30bp kuma girman 10kb.
◮Babban ingancin farfadowa:Mafi girman ingancin farfadowa zai iya kaiwa fiye da 80%.
◮Karamin tsarin haske:aƙalla 30μl bayani na elution za a iya amfani da shi don elution, wanda zai iya ƙara yawan ƙwayar DNA da aka gano.
◮Gudun sauri:Sauƙi don aiki, ana iya kammala dawo da guntun DNA a cikin mintuna 15.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:Rubutun DNA da aka dawo dasu suna da tsafta, waɗanda zasu iya saduwa da gwaje-gwaje daban-daban na gaba.
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EndoFree Maxi Plasmid Kit (Spin Column)
Duk aikin hakar yana ɗaukar awa 1 kawai, yana tabbatar da dacewa da aiki mai sauri.
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