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Zebra Kifi Kai tsaye PCR Kit
Wannan kayan aikin yana amfani da tsarin kariya na lysis na musamman don sakin DNA na jini daga zebrafish da sauran kayan kifin ruwa, wutsiyar wutsiya ko kwai kifayen samfuran PCR, saboda haka ya dace musamman don gwajin kwayar halitta mai girma. Hanyar sakewa da kwayoyin halittar Halitta (DNA) daga buhunan lysis an kammala shi tsakanin minti 10-30 a 65 ° C. Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma ana iya amfani da DNA ɗin da aka sake a matsayin samfuri don aikin PCR.
2 × PCR Easy TM Mix yana da juriya mai karfi ga masu hanawa PCR dauki, kuma zai iya amfani da lysate din samfurin don a gwada shi azaman samfuri don ingantaccen da takamaiman fadadawa. Wannan reagent din ya kunshi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, amsa maganin, PCR optimizer da stabilizer. An yi amfani da shi tare tare da man shafawa na lysis yana iya gano samfuran cikin sauri da sauƙi, kuma yana da halaye na ƙwarewa mai ƙarfi, ƙayyadaddun bayanai, da kwanciyar hankali mai kyau.
D-Taq DNA polymerase shine polymerase na DNA wanda aka haɓaka ta musamman ta Foregene don halayen PCR kai tsaye. D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'o'in masu hana PCR, kuma zai iya inganta haɓakar DNA sosai a cikin tsarin rikice-rikice masu rikitarwa, kuma saurin haɓaka zai iya isa 2Kb / min, wanda ya dace musamman don aikin PCR kai tsaye.
