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Naman Dabba Kai tsaye PCR kit-UNG
Wannan kayan aikin yana amfani da tsarin kariya na lysis na musamman don saurin sakin DNA daga samfuran nama na dabbobin don halayen PCR, saboda haka ya dace musamman don gwajin kwayar halitta mai girma.
Hanyar sakewa da kwayoyin halittar Halitta (DNA) daga buhunan lysis an kammala shi tsakanin minti 10-30 a 65°C. Ba a buƙatar wasu matakai kamar furotin da cire RNA, kuma ana iya amfani da DNA da aka sake a matsayin samfuri don aikin PCR.
2×PCR Mai SauƙiTM Mix (UNG) yayi amfani da dUTP maimakon dTTP akan 2×PCR Mai SauƙiTM Haɗa, kuma ƙara enzyme na UNG (Uracil-N-glycosylase) wanda zai iya lalata samfurin da ke ƙunshe da DUTP a lokaci guda. Kafin aikin PCR, ana amfani da enzyme na UNG don kaskantar da kayan PCR wanda ke dauke da uracil. UNG enzyme ba zai sami wani tasiri akan samfurin da ba ya ƙunshe da uracil ba, don haka tabbatar da ƙayyadaddun bayanai da daidaito na haɓakawa da hana yiwuwar gurɓata kayan PCR a yayin gwajin kwayar halitta mai girma.
D-Taq DNA polymerase shine polymerase na DNA wanda aka haɓaka ta musamman ta Foregene don halayen PCR kai tsaye. D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'o'in masu hana PCR, kuma zai iya inganta haɓakar DNA sosai a cikin tsarin rikice-rikice masu rikitarwa, kuma saurin haɓaka zai iya isa 2Kb / min, wanda ya dace musamman don aikin PCR kai tsaye.
