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Kayan Dabba Direct PCR kit
Wannan kayan aikin yana amfani da tsarin kariya na lysis na musamman don saurin sakin DNA daga samfuran nama na dabbobin don halayen PCR, saboda haka ya dace musamman don gwajin kwayar halitta mai girma.
Hanyar sakewa da kwayoyin halittar Halitta (DNA) daga buhunan lysis an kammala shi tsakanin minti 10-30 a 65°C. Ba a buƙatar wasu matakai kamar furotin da cire RNA, kuma ana iya amfani da DNA da aka sake a matsayin samfuri don aikin PCR.
2× PCR Mai SauƙiTM Mix yana da juriya mai ƙarfi ga masu hanawa na PCR, kuma zai iya amfani da lysate ɗin samfurin don a gwada shi azaman samfuri don ingantaccen da takamaiman haɓakawa. Wannan reagent yana dauke da Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, buffer mai amsawa, mai inganta yanayin PCR da karfafawa.
D-Taq DNA polymerase shine polymerase na DNA wanda aka haɓaka ta musamman ta Foregene don halayen PCR kai tsaye. D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'o'in masu hana PCR, kuma zai iya inganta haɓakar DNA sosai a cikin tsarin rikice-rikice masu rikitarwa, kuma saurin haɓaka zai iya isa 2Kb / min, wanda ya dace musamman don aikin PCR kai tsaye.
